FlyBase Allele Report: Dmel\CG32576[+t5.0]

General Information

Symbol

Dmel\CG32576^+t5.0

Species

D. melanogaster

Name

FlyBase ID

FBal0304571

Feature type

allele

Associated gene

Dmel\CG32576

Associated Insertion(s)

Carried in Construct

P{CG32576.+t5.0}

Key Links

Transgenic product class

genomic_DNA

(Wang et al., 2011)

wild_type

(Wang et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

genomic_DNA

(Wang et al., 2011)

wild_type

(Wang et al., 2011)

Mutagen

in vitro construct

(Wang et al., 2011)

Progenitor genotype

Carried in construct

P{CG32576.+t5.0}

Cytology

Description

5.0kb genomic fragment from the BAC CH322-46G07 clone. The fragment contains the CG32576 gene (but does not contain the neighbouring caz gene).

(Wang et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

CG32576

Encoded product / tool

CG32576

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight, with caz³⁸³

(Wang et al., 2011)

abnormal locomotor behavior, with caz³⁸³

(Wang et al., 2011)

abnormal neurophysiology, with caz³⁸³

(Shahidullah et al., 2013)

abnormal neurophysiology | third instar larval stage, with caz³⁸³

(Machamer et al., 2014)

partially lethal - majority die, with caz³⁸³

(Wang et al., 2012, Wang et al., 2011)

short lived, with caz³⁸³

(Wang et al., 2011)

visible, with caz³⁸³

(Wang et al., 2011)

Phenotype Manifest In

chaeta, with caz³⁸³

(Wang et al., 2011)

eye, with caz³⁸³

(Wang et al., 2011)

male genitalia, with caz³⁸³

(Wang et al., 2011)

wing vein, with caz³⁸³

(Wang et al., 2011)

Detailed Description

Statement

Reference

Male larvae hemizygous for the 'caz[1]' mutant chromosome (Df(1)383 onto which a transgene containing CG32576^+t5.0 has been recombined to provide CG32576[+] function) are morphologically normal. However, only 14% of these animals eclose as adults.

caz[1]/Y adult escapers show a 57% decrease in average life span compared to controls. They also show a number of external defects, including a mild rough eye phenotype, abnormal genitalia and defects in both bristles and wing vein organisation.

caz[1]/Y adult escapers have prominent locomotion defects. Most are unable to fly, and compared to controls, they walk slowly, often fall over, and have difficulty righting themselves. They show a 55.9% decrease in climbing ability in a negative geotaxis assay abd a 67.6% decrease in locomotor speed compared to controls.

The brains of 25 day old caz[1]/Y adult escapers do not show evidence of vacuolization or other evidence of extensive neuronal death.

(Wang et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

CG32576^+t5.0, caz³⁸³ has short lived phenotype, suppressible | partially by Scer\GAL4^elav-C155/Hsap\FUS^{P525L.10x.UAS.Tag:FLAG}

(Wang et al., 2011)