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General Information
Symbol
Dmel\Pol32R2
Species
D. melanogaster
Name
FlyBase ID
FBal0304627
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:

2015 bp deletion resulting from the imprecise excision of the progenitor insertion. Genome coordinates of deletion reported by author.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the progenitor P{PZ}Pol321006 insertion, resulting in a 2015bp deletion that removes nearly all of the Pol32 coding sequence and the 5' neighbouring l(2)35Cc gene.

Caused by aberration
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Pol32NR42/Pol32R2 females have short, thin bristles and moderate etching of the abdominal tergites. Mutant males have a more severe bristle and abdominal tergite phenotype.

Pol32NR42/Pol32R2 females lay eggs with an apparently normal chorion morphology, but they do not hatch. Embryos collected in a four hour period fall into three phenotypic classes: 11% have 1 nucleus, having a polar body with a star-like structure with additional or fragmented chromosomes, 57% arrest in mitotic cycles 1 to 6 with uneven distribution of nuclei and 31% appear to be degenerated, having no detectable chromatin.

Mitotic cells in Pol32NR42/Pol32R2 larval brains show a higher frequency of spontaneous chromosome breakage compared to controls. Three hours after treatment with 2.5 Gy X rays, more than 50% of mutant cells show chromosome breaks, a considerably higher proportion than control cells.

Pol32NR42/Pol32R2 animals are hypersensitive to ethyl methanesulphonate and to N-ethyl-N-nitrosourea compared to wild type.

Pol32NR42/Pol32R2 suppresses position effect variegation at the w locus caused by In(1)wm4h, but does not affect telomere position effect (TPE) in insertion lines carrying a w[+] marker.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT suppressed by
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The types and frequency of defects seen in embryos derived from Pol32NR42/Pol32R2 females is not altered if the females are also homozygous for mei-W68k05603.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Pol32R2
Name Synonyms
Secondary FlyBase IDs
    References (1)