Open Close
General Information
Symbol
Dmel\mir-318Δ1
Species
D. melanogaster
Name
FlyBase ID
FBal0304752
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

A 126bp genomic fragment including the mir-318 hairpin has been deleted and replaced by sequence encoding EGFP and the w+mW.hs marker. This places EGFP is under the control of the mir-318 regulatory elements.

Insertion components
TI{EGFP}mir-318Δ1
Product class / Tool use(s)
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

mir-318Δ1/mir-318Δ2 mutant flies are fully viable and do not show obvious morphological defects. However, mir-318Δ1/mir-318Δ2 mutant females lay eggs with a range of defects in eggshell formation and patterning. More than 90% of eggs from mir-318Δ1/mir-318Δ2 mutant mothers show defects in dorsal pattern: the dorsal appendages are shifted towards posterior and are closer together than in wild type embryos. The operculum is slightly enlarged.

76% of eggs from mir-318Δ1/mir-318Δ2 mutant mothers fail to hatch into first instar larvae. Most of the eggs appear not to be fertilized, and the micropyle is reduced or absent. Eggs also have a transparent and thin eggshell. 12% of eggs collapse by 48 hours after laying.

mir-318Δ1/mir-318Δ2 mutants females show defects in follicle cell patterning. The transition from endocycles to chorion gene amplification is compromised in mir-318Δ1/mir-318Δ2 mutant follicle cells.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement
Reference

mir-318Δ1/mir-318Δ2 has egg phenotype, enhanceable by ttk[+]/ttk1e11

mir-318Δ1/mir-318Δ2 has egg phenotype, enhanceable by Df(3R)ED6361/+

Additional Comments
Genetic Interactions
Statement
Reference

One copy of ttk1e11 enhances the abnormal egg phenotype seen in mir-318Δ1/mir-318Δ2 mutants, including short dorsal appendages. None of the mir-318Δ1 mir-318Δ2/ttk1e11 mutant flies are able to hatch into first instar larval females. A more severe reduction in chorion gene amplification is seen.

One copy of Df(3R)ED6361 enhances the abnormal egg phenotype seen in mir-318Δ1/mir-318Δ2 mutants, including short dorsal appendages. None of the mir-318Δ1 mir-318Δ2/Df(3R)ED6361 mutant flies are able to hatch into first instar larval females.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of mir-318+tGa rescues the defects seen in eggs laid by mir-318Δ1/mir-318Δ2 mutant mothers. The hatching rate is restored to wild type levels.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
mir-318Δ1
Name Synonyms
Secondary FlyBase IDs
    References (1)