FlyBase Allele Report: Dmel\mir-969[sponge.V2.UAS.mCherry]

General Information

Symbol

Dmel\mir-969^{sponge.V2.UAS.mCherry}

Species

D. melanogaster

Name

FlyBase ID

FBal0304874

Feature type

allele

Associated gene

Dmel\mir-969

Associated Insertion(s)

Carried in Construct

P{UAS-mCherry.mir-969.sponge.V2}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Van Vactor, 2015.6.15)

Progenitor genotype

Carried in construct

P{UAS-mCherry.mir-969.sponge.V2}

Cytology

Description

Twenty miRNA binding sites with mismatches at positions 9-12 (creating a "Sponge", designed to sequester miRNAs and thus knocking-down endogenous miRNA activity) are placed in the 3'UTR of mCherry under the control of 10 UAS binding sites.

(Van Vactor, 2015.6.15)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

mir-969

Tagged with

mCherry

red fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^mir-969.KO

(Zhang et al., 2020)

abnormal locomotor behavior | adult stage, with Scer\GAL4^Toll-6-D42

(Zhang et al., 2020)

abnormal locomotor behavior | adult stage, with Scer\GAL4^VGlut1-OK371

(Zhang et al., 2020)

viable, with Scer\GAL4^Tub.PU

(Fulga et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

mir-969^{sponge.V2.UAS.mCherry}/Scer\GAL4^αTub84B.PL is a non-suppressor of lethal | recessive phenotype of wupA²³⁴³⁷

(Casas-Tintó and Ferrús, 2021)

mir-969^{sponge.V2.UAS.mCherry}/Scer\GAL4^αTub84B.PL is a non-suppressor of lethal | recessive phenotype of wupA^hdp-3

(Casas-Tintó and Ferrús, 2021)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

61455

w^*; P{UAS-mCherry.mir-969.sponge.V2}attP40/CyO; P{UAS-mCherry.mir-969.sponge.V2}attP2/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

mir-969^{sponge.V2.Scer\UAS.T:Disc\RFP-mCherry}

mir-969^{sponge.V2.UAS.mCherry}

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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