FlyBase Allele Report: Dmel\mir-11[sponge.V2.UAS.mCherry]

General Information

Symbol

Dmel\mir-11^{sponge.V2.UAS.mCherry}

Species

D. melanogaster

Name

FlyBase ID

FBal0304980

Feature type

allele

Associated gene

Dmel\mir-11

Associated Insertion(s)

Carried in Construct

P{UAS-mCherry.mir-11.sponge.V2}

Allele class

Mutagen

in vitro construct - regulatory fusion

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Fulga et al., 2015)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{UAS-mCherry.mir-11.sponge.V2}

Cytology

Nature of the lesion

Statement

Reference

Twenty miRNA binding sites with mismatches at positions 9-12 (creating a "Sponge", designed to sequester miRNAs and thus knocking-down endogenous miRNA activity) are placed in the 3'UTR of mCherry under the control of 10 Gal4 UAS binding sites.

(Fulga et al., 2015)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

mir-11

Tagged with

mCherry

red fluorescent protein

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

viable, with Scer\GAL4^Tub.PU

(Fulga et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

61378

w^*; P{UAS-mCherry.mir-11.sponge.V2}attP40; P{UAS-mCherry.mir-11.sponge.V2}attP2

Notes on Origin

Discoverer

Comments

The lack of a Scer\GAL4^tub.PU-mediated viability phenotype is a false negative (compared to independent viability data from miRNA deletion mutants).

(Fulga et al., 2015)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

mir-11^{sponge.V2.Scer\UAS.T:Disc\RFP-mCherry}

mir-11^{sponge.V2.UAS.mCherry}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

Open Close