Twenty miRNA binding sites with mismatches at positions 9-12 (creating a "Sponge", designed to sequester miRNAs and thus knocking-down endogenous miRNA activity) are placed in the 3'UTR of mCherry under the control of 10 Gal4 UAS binding sites.
viable, with Scer\GAL4Tub.PU
mir-184sponge.V2.UAS.mCherry, Scer\GAL4ap.PU is an enhancer of increased cell death phenotype of GliUAS.cSa, Scer\GAL4ap.PU
mir-184sponge.V2.UAS.mCherry, Scer\GAL4ap.PU is an enhancer of wing disc phenotype of GliUAS.cSa, Scer\GAL4ap.PU
Co-expression of two copies of mir-184sponge.V2.Scer\UAS.T:Disc\RFP-mCherry enhances the migration of wing disc cells expressing GliScer\UAS.cSa under the control of Scer\GAL4ap.PU into the non-expressing side of the disc.