FB2021_01, released Feb 18, 2021

Allele: Dmel\mir-276aa.sponge.V2.UAS.mCherry

Open Close
General Information
Symbol
Dmel\mir-276aa.sponge.V2.UAS.mCherry
Species
D. melanogaster
Name
FlyBase ID
FBal0305006
Feature type
allele
Associated gene
Dmel\mir-276a
Associated Insertion(s)
Carried in Construct
P{UAS-mCherry.mir-276a.sponge.V2}
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - regulatory fusion
(Fulga et al., 2015)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{UAS-mCherry.mir-276a.sponge.V2}
      Cytology
      Nature of the lesion
      Statement
      Reference

      The P{UAS-mCherry.mir-276a.sponge.V2} and P{UAS-mCherry.mir-276b.sponge.V2} constructs target mir-276a-3P and mir-276b-3P respectively. However, because the only difference between mir-276a-3P and mir-276b-3P is at position 10, and position 10 is in the sponge bulge (not complementary to the miRNA), both sponges target both miRNAs.

      (Cook and Van Vactor, 2015.6.15)

      Twenty miRNA binding sites with mismatches at positions 9-12 (creating a "Sponge", designed to sequester miRNAs and thus knocking-down endogenous miRNA activity) are placed in the 3'UTR of mCherry under the control of 10 Gal4 UAS binding sites.

      (Fulga et al., 2015)
      Allele components
      Product class / Tool use(s)
      Regulatory region(s)
      UAS
      Encoded product / tool
      mir-276a
      Tagged with
      mCherry
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Adult-onset expression of mir-276aa.sponge.V2.Scer\UAS.T:Disc\RFP-mCherry under the control of Scer\GAL4Pdf.Switch1a (used with RU486-supplemented diet to activate the expression in adult flies only) results in arrhythmic circadian activity in 44% of adult flies. Circadian free-running period in the mutant flies that remain (at least partially) rhythmic is higher than in controls, but most likely just due to inaccuracy of the measurements caused by the decreased rhythmicity.

      (Chen and Rosbash, 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      The lack of a Scer\GAL4tub.PU-mediated viability phenotype is a false negative (compared to independent viability data from miRNA deletion mutants).

      (Fulga et al., 2015)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      mir-276aa.sponge.V2.Scer\UAS.T:Disc\RFP-mCherry
      mir-276aa.sponge.V2.UAS.mCherry
      mir-276asponge.V2.Scer\UAS.T:Disc\RFP-mCherry
      Name Synonyms
      Secondary FlyBase IDs
        References (3)