FB2025_01 , released February 20, 2025
Allele: Dmel\spagRNAi.UAS.cUa
Open Close
General Information
Symbol
Dmel\spagRNAi.UAS.cUa
Species
D. melanogaster
Name
FlyBase ID
FBal0314186
Feature type
allele
Associated gene
Dmel\spag
Associated Insertion(s)
Carried in Construct
P{UAS-spag.RNAi.U}
Key Links
Transgenic product class
RNAi_reagent
(Means et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Means et al., 2015)
Mutagen
in vitro construct
(Means et al., 2015)
Progenitor genotype
Carried in construct
P{UAS-spag.RNAi.U}
Cytology
Description

UAS regulatory sequences drive expression of an RNAi construct targeting spag.

(Means et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
spag
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of spagdsRNA.Scer\UAS.cUa in clock cells under the control of Scer\GAL4tim.PE leads to altered locomotor activity rhythms relative to wild-type. The spagdsRNA.Scer\UAS.cUa-expressing flies exhibit long periods (5.5-26.5 h) or arrhythmic locomotor activity depending on the specific RNAi transgene, the inclusion of Dcr-2 in the genotype and the duration of transgene expression after eclosion.

      Flies expressing Scer\GAL4tim.PE>spagdsRNA.Scer\UAS.cUa show circadian-dependent activation of apoptosis in the head at an earlier age than do wild-type flies.

      Flies expressing Scer\GAL4tim.PE>spagdsRNA.Scer\UAS.cUa initially climb as well as control flies. However, over time they decline in performance more rapidly than controls. In addition, the Scer\GAL4tim.PE>spagdsRNA.Scer\UAS.cUa flies show reduced lifespan compared with controls.

      (Means et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      spagRNAi.UAS.cUa
      spagdsRNA.Scer\UAS.cUa
      spagdsRNA.UAS.cUa
      Name Synonyms
      Secondary FlyBase IDs
        References (2)