FlyBase Allele Report: Dmel\DAAM[C.UASp]

General Information

Symbol

Dmel\DAAM^C.UASp

Species

D. melanogaster

Name

FlyBase ID

FBal0314316

Feature type

allele

Associated gene

Dmel\DAAM

Associated Insertion(s)

Carried in Construct

P{UASp-DAAM.C}

Also Known As

UAS-C-DAAM

Key Links

Transgenic product class

inframe_deletion

(Matusek et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Matusek et al., 2006)

Mutagen

in vitro construct

(Matusek et al., 2006)

Progenitor genotype

Carried in construct

P{UASp-DAAM.C}

Cytology

Description

UASp regulatory sequences drive expression of amino acids 518-1153 of DAAM. This is produces an activated form of DAAM.

(Matusek et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

DAAM

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^elav-C155

(Matusek et al., 2008)

abnormal neuroanatomy, with Scer\GAL4^ey-OK107

(Gombos et al., 2015)

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^elav.PLu

(Földi et al., 2022)

lethal - all die during embryonic stage, with Scer\GAL4^elav-C155

(Matusek et al., 2008)

some die during embryonic stage, with Scer\GAL4^elav-C155

(Matusek et al., 2008)

Phenotype Manifest In

axon microtubule bundle | embryonic stage, with Scer\GAL4^elav.PLu

(Földi et al., 2022)

embryonic/larval motor neuron | embryonic stage, with Scer\GAL4^elav.PLu

(Földi et al., 2022)

fascicle | embryonic stage, with Scer\GAL4^elav-C155

(Matusek et al., 2008)

filamentous actin & tracheal fusion cell, with DAAM^UASp.cMa, Scer\GAL4^btl.PS

(Matusek et al., 2006)

filamentous actin & tracheal fusion cell, with Scer\GAL4^btl.PS

(Matusek et al., 2006)

filamentous actin & tracheolar cell, with Scer\GAL4^btl.PS

(Matusek et al., 2006)

mushroom body, with Scer\GAL4^ey-OK107

(Gombos et al., 2015)

neuropil | embryonic stage, with Scer\GAL4^elav-C155

(Matusek et al., 2008)

symmetrical commissure | embryonic stage, with Scer\GAL4^elav-C155

(Matusek et al., 2008)

Detailed Description

Statement

Reference

Expression of DAAM^{C.Scer\UAS.P\T} under the control of Scer\GAL4^ey-OK107 induces very severe growth and guidance defects in the mushroom body axons Unlike in wild type, these axons fail to project anteriorly and form a huge, unstructured, randomly oriented network of projections with very prominent but misprojected bundles in the posterior region of the brain.

(Gombos et al., 2015)

Expression of DAAM^{C.Scer\UAS.P\T} pan-neuronally, under the control of Scer\GAL4^elav-C155, results in severe fasciculation defects and embryonic lethality. The majority of embryos (78%) display a more prominent neuropile than observed in wild-type, commissures and nerve roots in particular appear thicker. Many axons are misrouted, and either cross the midline or exit the bundles into lateral directions.

(Matusek et al., 2008)

Expression of DAAM^{C.Scer\UAS.P\T} in the trachea, driven by Scer\GAL4^btl.PS, causes distinct phenotypic effects in the fusion cells and the regular tracheal cells. In fusion cells of this genotype, there is a dramatic change in the actin organization and cuticle pattern, causing strong actin accumulation at the apical surface. The typical granular cuticle pattern is transformed towards a stripy pattern , partly resembling the taenidial folds of normal tracheal cells. Most of the actin in these cells is found in largely unorganized bundles. In tracheal cells, DAAM^{C.Scer\UAS.P\T} overexpression results in severe impairment of taenidial fold formation and actin organization.

Coexpression of DAAM^{Scer\UAS.P\T.cMa} and DAAM^{C.Scer\UAS.P\T}, under the control of Scer\GAL4^btl.PS, causes a suppression of the Scer\GAL4^btl.PS/DAAM^{C.Scer\UAS.P\T} phenotype in regular tracheal cells but not in fusion cells.

(Matusek et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

DAAM^C.UASp, Scer\GAL4^elav-C155 has abnormal neuroanatomy phenotype, non-enhanceable by Rac1^J11

(Matusek et al., 2008)

DAAM^C.UASp has abnormal neuroanatomy phenotype, non-enhanceable by chic[+]/chic²²¹

(Matusek et al., 2008)

DAAM^C.UASp, Scer\GAL4^elav-C155 has abnormal neuroanatomy phenotype, non-enhanceable by Rac1^J11/Rac1^J10

(Matusek et al., 2008)

DAAM^C.UASp, Scer\GAL4^elav-C155 has abnormal neuroanatomy phenotype, non-enhanceable by Rac1^J11/Rac2^Δ

(Matusek et al., 2008)

DAAM^C.UASp, Scer\GAL4^elav-C155 has abnormal neuroanatomy phenotype, non-enhanceable by Rac1^J11/Rac2^Δ/Mtl^Δ

(Matusek et al., 2008)

Suppressed by

Statement

Reference

DAAM^C.UASp has abnormal neuroanatomy phenotype, suppressible by ena²³/ena[+]

(Matusek et al., 2008)

DAAM^C.UASp has abnormal neuroanatomy phenotype, suppressible by ena[+]/ena^GC1

(Matusek et al., 2008)

NOT suppressed by

Statement

Reference

DAAM^C.UASp, Scer\GAL4^ey-OK107 has abnormal neuroanatomy phenotype, non-suppressible by dsh¹/dsh[+]

(Gombos et al., 2015)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

DAAM^C.UASp, Scer\GAL4^elav-C155 has neuropil | embryonic stage phenotype, non-enhanceable by Rac1^J11

(Matusek et al., 2008)

DAAM^C.UASp, Scer\GAL4^elav-C155 has neuropil | embryonic stage phenotype, non-enhanceable by Rac1^J11/Rac2^Δ/Mtl^Δ

(Matusek et al., 2008)

DAAM^C.UASp, Scer\GAL4^elav-C155 has fascicle | embryonic stage phenotype, non-enhanceable by Rac1^J11/Rac2^Δ/Mtl^Δ

(Matusek et al., 2008)

DAAM^C.UASp, Scer\GAL4^elav-C155 has symmetrical commissure | embryonic stage phenotype, non-enhanceable by Rac1^J11/Rac2^Δ/Mtl^Δ

(Matusek et al., 2008)

DAAM^C.UASp, Scer\GAL4^elav-C155 has fascicle | embryonic stage phenotype, non-enhanceable by Rac1^J11/Mtl^Δ

(Matusek et al., 2008)

DAAM^C.UASp, Scer\GAL4^elav-C155 has neuropil | embryonic stage phenotype, non-enhanceable by Rac1^J11/Rac1^J10, Scer\GAL4^elav-C155

(Matusek et al., 2008)

DAAM^C.UASp, Scer\GAL4^elav-C155 has fascicle | embryonic stage phenotype, non-enhanceable by Rac1^J11/Mtl^Δ/Rac1^J10

(Matusek et al., 2008)

DAAM^C.UASp, Scer\GAL4^elav-C155 has neuropil | embryonic stage phenotype, non-enhanceable by chic[+]/chic²²¹

(Matusek et al., 2008)

DAAM^C.UASp, Scer\GAL4^elav-C155 has fascicle | embryonic stage phenotype, non-enhanceable by chic[+]/chic²²¹

(Matusek et al., 2008)

DAAM^C.UASp, Scer\GAL4^elav-C155 has symmetrical commissure | embryonic stage phenotype, non-enhanceable by chic[+]/chic²²¹

(Matusek et al., 2008)

DAAM^C.UASp, Scer\GAL4^elav-C155 has symmetrical commissure | embryonic stage phenotype, non-enhanceable by Rac1^J11

(Matusek et al., 2008)

DAAM^C.UASp, Scer\GAL4^elav-C155 has symmetrical commissure | embryonic stage phenotype, non-enhanceable by Rac1^J11/Rac1^J10

(Matusek et al., 2008)

DAAM^C.UASp, Scer\GAL4^elav-C155 has neuropil | embryonic stage phenotype, non-enhanceable by Rac1^J11/Rac2^Δ

(Matusek et al., 2008)

DAAM^C.UASp, Scer\GAL4^elav-C155 has fascicle | embryonic stage phenotype, non-enhanceable by Rac1^J11/Rac2^Δ

(Matusek et al., 2008)

DAAM^C.UASp, Scer\GAL4^elav-C155 has symmetrical commissure | embryonic stage phenotype, non-enhanceable by Rac1^J11/Rac2^Δ

(Matusek et al., 2008)

Suppressed by

Statement

Reference

DAAM^C.UASp, Scer\GAL4^elav-C155 has fascicle | embryonic stage phenotype, suppressible by ena[+]/ena^GC1

(Matusek et al., 2008)

DAAM^C.UASp, Scer\GAL4^elav-C155 has symmetrical commissure | embryonic stage phenotype, suppressible by ena[+]/ena^GC1

(Matusek et al., 2008)

DAAM^C.UASp, Scer\GAL4^elav-C155 has neuropil | embryonic stage phenotype, suppressible by ena²³/ena[+]

(Matusek et al., 2008)

DAAM^C.UASp, Scer\GAL4^elav-C155 has fascicle | embryonic stage phenotype, suppressible by ena²³/ena[+]

(Matusek et al., 2008)

DAAM^C.UASp, Scer\GAL4^elav-C155 has symmetrical commissure | embryonic stage phenotype, suppressible by ena²³/ena[+]

(Matusek et al., 2008)

DAAM^C.UASp, Scer\GAL4^btl.PS has embryonic/larval tracheal section phenotype, suppressible by Src42A^E1

(Matusek et al., 2006)

DAAM^C.UASp, Scer\GAL4^btl.PS has embryonic/larval tracheal section phenotype, suppressible by Btk^k05610

(Matusek et al., 2006)

NOT suppressed by

Statement

Reference

DAAM^C.UASp, Scer\GAL4^ey-OK107 has mushroom body phenotype, non-suppressible by dsh¹/dsh[+]

(Gombos et al., 2015)

DAAM^C.UASp, Scer\GAL4^btl.PS has embryonic/larval tracheal section phenotype, non-suppressible by Rho1^72F

(Matusek et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

dsh¹ does not suppress the growth and guidance defects seen in the mushroom body axons of flies expressing DAAM^{C.Scer\UAS.P\T} under the control of Scer\GAL4^ey-OK107.

(Gombos et al., 2015)

The Scer\GAL4^elav-C155/DAAM^{C.Scer\UAS.P\T} gain-of-function phenotype (i.e the appearance of thicker commissures and nerve roots) is not affected by Rac1 gene dose (i.e. a Rac1^J11/+ or Rac1^J11/Rac1^J10 background), a Rac1^J11, Rac2^Δ background or a Rac1^J10, Rac2^Δ, Mtl^Δ background.

The Scer\GAL4^elav-C155/DAAM^{C.Scer\UAS.P\T} gain-of-function phenotype (i.e the appearance of thicker commissures and nerve roots) is not affected by a chic²²¹/+ background.

The Scer\GAL4^elav-C155/DAAM^{C.Scer\UAS.P\T} gain-of-function phenotype (i.e the appearance of thicker commissures and nerve roots) is suppressed by a ena²³/+ or ena^GC1/+ background.

(Matusek et al., 2008)

The tracheal cuticle defects of larvae that express DAAM^{C.Scer\UAS.P\T} under the control of Scer\GAL4^btl.PS are dominantly suppressed by Src42A^E1 and Btk29A^k05610. However, Rho1^72F does not modify the effect of modify the effect of DAAM^{C.Scer\UAS.P\T} expression.

(Matusek et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

DAAM^{C.Scer\UAS.P\T}

DAAM^C.UASp

DAAM^CA.Scer\UAS

Name Synonyms

Secondary FlyBase IDs

FBal0194065
FBal0240537

References (7)

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