FlyBase Allele Report: Dmel\Neto[204]

General Information

Symbol

Dmel\Neto²⁰⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0314786

Feature type

allele

Associated gene

Dmel\Neto

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

minos activity

Nature of the Allele

Allele class

Mutagen

minos activity

(Ramos et al., 2015)

Progenitor genotype

Mi{ET1}Neto^MB07125

(Ramos et al., 2015)

Cytology

Description

Imprecise excision of Mi{ET1}Neto^MB07125 has resulted in a deletion that removes the entire Neto β isoform-specific sequence ( X:13 ,520,444..13,523,898 (R6)). This comprises exons 12, 13 and 14.

(Ramos et al., 2015)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage

(Ramos et al., 2015)

abnormal locomotor behavior | larval stage

(Ramos et al., 2015)

abnormal neurophysiology

(Ramos et al., 2015)

lethal (with Neto³⁶)

(Ramos et al., 2015)

some die during embryonic stage

(Ramos et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

Neto²⁰⁴ mutant adult flies have impaired climbing ability.

The spontaneous miniature excitatory junctional potential (mEJP) amplitude and frequency are reduced in Neto²⁰⁴ mutant third instar larvae. Evoked EJP amplitude is similar to controls. Quantal content is increased compared to controls.

Neto²⁰⁴ mutant NMJs are reduced in length compared with controls and have shorter branches. The Neto²⁰⁴ mutant NMJS have fewer but larger type Ib boutons. The enlarged Neto²⁰⁴ mutant boutons have diminished or absent subsynaptic reticulum (SSR) structures.

Neto²⁰⁴ mutant NMJs have an increased number of synaptic contacts, and their density is increased at proximal but not distal boutons.

The active zones of Neto²⁰⁴ mutant NMJs often have abnormal T-bar structures. Postsynaptic density (PSD) length is significantly reduced compared to controls.

(Ramos et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Dp(1;3)DC270 rescues the lethality seen in Neto³⁶/Neto²⁰⁴ mutant flies.

(Ramos et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Neto²⁰⁴

neto-β^null

(Han et al., 2020)

neto²⁰⁴

(Ramos et al., 2015)

neto^βnull

(Ramos et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

Open Close