FlyBase Allele Report: Dmel\robo1[10xUAS.Tag:HA,Tag:SS(wg)]

General Information

Symbol

Dmel\robo1^{10xUAS.Tag:HA,Tag:SS(wg)}

Species

D. melanogaster

Name

FlyBase ID

FBal0316444

Feature type

allele

Associated gene

Dmel\robo1

Associated Insertion(s)

Carried in Construct

M{10xUAS-HA-robo1}

Also Known As

UAS-Robo1, P{10UAS-HARobo1}

Key Links

Transgenic product class

wild_type

(Evans and Bashaw, 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Evans and Bashaw, 2012)

Mutagen

in vitro construct

(Evans et al., 2015, Evans and Bashaw, 2012)

Progenitor genotype

Carried in construct

M{10xUAS-HA-robo1}

Cytology

Description

Ten copies of the UAS regulatory sequence drive expression of the wild-type robo1 coding sequence which is tagged at the N-terminal end with three copies of the Tag:HA tag. 5'UTR and signal sequences from the wg gene are also present.

(Evans et al., 2015)

UASt regulatory sequences are fused upstream of the wg 5'UTR and signal sequence, three copies of the Tag:HA tag and the robo1 coding sequence.

(Evans and Bashaw, 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

UASt

binary expression system - regulatory region

Encoded product / tool

Tagged with

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

commissure | embryonic stage, with Scer\GAL4^elav.PLu

(Daiber et al., 2021)

embryonic midline of ventral nerve cord, with Scer\GAL4^elav.PLu

(Daiber et al., 2021)

larval EW neuron, with Scer\GAL4^eg-Mz360

(Evans et al., 2015, Evans and Bashaw, 2012)

larval EW neuron | embryonic stage, with Scer\GAL4^eg-Mz360

(Daiber et al., 2021, Brown et al., 2015)

larval longitudinal connective, with Scer\GAL4^sli.PS

(Evans et al., 2015)

larval longitudinal connective | embryonic stage, with Scer\GAL4^elav.PLu

(Brown et al., 2015)

larval ventral nerve cord | embryonic stage, with Scer\GAL4^eg-Mz360

(Brown et al., 2015)

larval ventral nerve cord | embryonic stage, with Scer\GAL4^elav.PLu

(Brown et al., 2015)

larval ventral nerve cord commissure | embryonic stage, with Scer\GAL4^elav.PLu

(Brown et al., 2015)

symmetrical commissure, with Scer\GAL4^elav.PLu

(Evans et al., 2015)

Detailed Description

Statement

Reference

Expression of robo1^{10xScer\UAS.T:Ivir\HA1,T:SS-wg} under the control of Scer\GAL4^elav.PLu in embryos results in strong inhibition of midline crossing in the ventral nerve cord, producing thin or absent commissures in nearly 100% of segments, and also results in disorganization of longitudinal axon pathways.

Expression of robo1^{10xScer\UAS.T:Ivir\HA1,T:SS-wg} under the control of Scer\GAL4^eg-Mz360 in embryos results in failure of EW neurons to cross the midline of the ventral nerve cord.

(Brown et al., 2015)

Commissure formation is strongly impaired in embryos expressing robo1^{10xScer\UAS.T:Ivir\HA1,T:SS-wg} under the control of Scer\GAL4^elav.PLu. No ectopic crossing of the midline by Fas2-positive axons is seen.

Expression of robo1^{10xScer\UAS.T:Ivir\HA1,T:SS-wg} under the control of Scer\GAL4^eg-Mz360 in embryos results in 97.1% of EW neurons failing to cross the midline.

Expression of robo1^{10xScer\UAS.T:Ivir\HA1,T:SS-wg} under the control of Scer\GAL4^sli.PS in embryos results in a mild ectopic midline crossing phenotype in the Fas2-positive axons.

(Evans et al., 2015)

Expression of robo1^{10xScer\UAS.T:Ivir\HA1,T:SS-wg} under the control of Scer\GAL4^eg-Mz360 in embryos results in the EW axons failing to cross the midline in almost all segments.

(Evans and Bashaw, 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

robo1^{10xUAS.Tag:HA,Tag:SS(wg)}/Scer\GAL4^elav.PLu rescues robo1¹

(Daiber et al., 2021)

robo1^{10xUAS.Tag:HA,Tag:SS(wg)}/Scer\GAL4^elav.PLu rescues robo1¹

(Evans and Bashaw, 2012)

Partially rescues

robo1^{10xUAS.Tag:HA,Tag:SS(wg)}/Scer\GAL4^elav.PLu partially rescues robo1¹

(Brown et al., 2015)

Comments

Expression of robo1^{10xScer\UAS.T:Ivir\HA1,T:SS-wg} under the control of Scer\GAL4^elav.PLu rescues the FasII-positive axon guidance defects of robo1¹/robo1¹ mutants, but also causes additional axon guidance defects, including disruption of normal commissure formation and disorganization of longitudinal axon pathways.

(Brown et al., 2015)

Expression of robo1^{10xScer\UAS.T:Ivir\HA1,T:SS-wg} under the control of Scer\GAL4^elav.PLu rescues the ectopic midline crossing phenotype seen in the medial Fas2-positive axons in robo1¹ embryos.

(Evans and Bashaw, 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

97238

w^*; M{10xUAS-HA-robo1}ZH-86Fb

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

robo1^{10xScer\UAS.T:Ivir\HA1,T:SS-wg}

robo1^{10xUAS.Tag:HA,Tag:SS(wg)}

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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