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General Information
Symbol
Dmel\robo110xUAS.Tag:HA,Tag:SS(wg)
Species
D. melanogaster
Name
FlyBase ID
FBal0316444
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
P{10UAS-HARobo1}, UAS-Robo1
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Ten copies of the UAS regulatory sequence drive expression of the wild-type robo1 coding sequence which is tagged at the N-terminal end with three copies of the Tag:HA tag. 5'UTR and signal sequences from the wg gene are also present.

UASt regulatory sequences are fused upstream of the wg 5'UTR and signal sequence, three copies of the Tag:HA tag and the robo1 coding sequence.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of robo110xScer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4elav.PLu in embryos results in strong inhibition of midline crossing in the ventral nerve cord, producing thin or absent commissures in nearly 100% of segments, and also results in disorganization of longitudinal axon pathways.

Expression of robo110xScer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4eg-Mz360 in embryos results in failure of EW neurons to cross the midline of the ventral nerve cord.

Commissure formation is strongly impaired in embryos expressing robo110xScer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4elav.PLu. No ectopic crossing of the midline by Fas2-positive axons is seen.

Expression of robo110xScer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4eg-Mz360 in embryos results in 97.1% of EW neurons failing to cross the midline.

Expression of robo110xScer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4sli.PS in embryos results in a mild ectopic midline crossing phenotype in the Fas2-positive axons.

Expression of robo110xScer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4eg-Mz360 in embryos results in the EW axons failing to cross the midline in almost all segments.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of robo110xScer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4elav.PLu rescues the FasII-positive axon guidance defects of robo11/robo11 mutants, but also causes additional axon guidance defects, including disruption of normal commissure formation and disorganization of longitudinal axon pathways.

Expression of robo110xScer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4elav.PLu rescues the ectopic midline crossing phenotype seen in the medial Fas2-positive axons in robo11 embryos.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
robo110xScer\UAS.T:Ivir\HA1,T:SS-wg
robo110xUAS.Tag:HA,Tag:SS(wg)
Name Synonyms
Secondary FlyBase IDs
    References (3)