FB2025_01 , released February 20, 2025
Allele: Tcas\roboUAS.Tag:HA,Tag:SS(wg)
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General Information
Symbol
Tcas\roboUAS.Tag:HA,Tag:SS(wg)
Species
T. castaneum
Name
FlyBase ID
FBal0316447
Feature type
allele
Associated gene
Tcas\robo
Associated Insertion(s)
Carried in Construct
M{UAS-HA-Tcrobo}
Transgenic product class
wild_type
(Evans and Bashaw, 2012)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Evans and Bashaw, 2012)
Mutagen
in vitro construct
(Evans and Bashaw, 2012)
Progenitor genotype
Carried in construct
M{UAS-HA-Tcrobo}
Cytology
Description

UASt regulatory sequences are fused upstream of the wg 5'UTR and signal sequence, three copies of the Tag:HA tag and the Tcas\robo coding sequence.

(Evans and Bashaw, 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Tcas\robo
Tagged with
Tag:HA
Tag:SS(wg)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Tcas\roboScer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4elav.PLu results in embryos with a commissureless phenotype.

      Expression of Tcas\roboScer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4eg-Mz360 in embryos results in the EW axons failing to cross the midline in almost all segments.

      (Evans and Bashaw, 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT suppressed by
      Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      The ectopic midline crossing phenotype seen in the medial Fas2-positive axons in robo11 embryos is suppressed if they are expressing Tcas\roboScer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4elav.PLu, with nearly all segments being commissureless in these animals.

      (Evans and Bashaw, 2012)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Tcas\roboScer\UAS.T:Ivir\HA1,T:SS-wg
      Tcas\roboUAS.Tag:HA,Tag:SS(wg)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)