FB2025_01 , released February 20, 2025
Allele: Dmel\robo2ΔIg2.10xUAS.Tag:HA,Tag:SS(wg)
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General Information
Symbol
Dmel\robo2ΔIg2.10xUAS.Tag:HA,Tag:SS(wg)
Species
D. melanogaster
Name
FlyBase ID
FBal0316476
Feature type
allele
Associated gene
Dmel\robo2
Associated Insertion(s)
Carried in Construct
M{10xUAS-HA-robo2.ΔIg2}
Key Links
Transgenic product class
inframe_deletion
(Evans et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Evans et al., 2015)
Mutagen
in vitro construct
(Evans et al., 2015)
Progenitor genotype
Carried in construct
M{10xUAS-HA-robo2.ΔIg2}
Cytology
Description

Ten copies of the UAS regulatory sequence drive expression of a deleted robo2 coding sequence (lacks amino acid residues 281-1463, numbering according to GenBank AAF51375) which is tagged at the N-terminal end with three copies of the Tag:HA tag. 5'UTR and signal sequences from the wg gene are also present.

(Evans et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
robo2
Tagged with
Tag:HA
Tag:SS(wg)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of robo2ΔIg2.10xScer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4eg-Mz360 in embryos results in 74% of EW neurons failing to cross the midline.

      Expression of robo2ΔIg2.10xScer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4sli.PS in embryos does not result in ectopic crossing of the midline by Fas2-positive axons.

      (Evans et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Suppressor of
      Phenotype Manifest In
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of robo2ΔIg2.10xScer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4elav.PLu does not suppress the loss of commissure phenotype of homozygous commE39 embryos.

      (Evans et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      robo2ΔIg2.10xScer\UAS.T:Ivir\HA1,T:SS-wg
      robo2ΔIg2.10xUAS.Tag:HA,Tag:SS(wg)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)