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General Information
Symbol
Dmel\robo2ΔC.10xUAS.Tag:HA,Tag:SS(wg)
Species
D. melanogaster
Name
FlyBase ID
FBal0316478
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Ten copies of the UAS regulatory sequence drive expression of a deleted robo2 coding sequence (lacks amino acid residues 84-1022, numbering according to GenBank AAF51375) which is tagged at the N-terminal end with three copies of the Tag:HA tag. 5'UTR and signal sequences from the wg gene are also present.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of robo2ΔC.10xScer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4elav.PLu in embryos results in ectopic crossing of the midline by Fas2-positive axons in 100% of segments. All three Fas2-positive pathways collapse at the midline in nearly every segment.

Expression of robo2ΔC.10xScer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4ap.PU in embryos results in low levels of ectopic crossing of the midline by ap-expressing neurons.

Expression of robo2ΔC.10xScer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4sli.PS in embryos results in ectopic crossing of the midline by Fas2-positive axons in almost all segments. Fused commissures are seen in approximately 80% of segments.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
robo2ΔC.10xScer\UAS.T:Ivir\HA1,T:SS-wg
robo2ΔC.10xUAS.Tag:HA,Tag:SS(wg)
Name Synonyms
Secondary FlyBase IDs
    References (1)