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General Information
Symbol
Dmel\RootUASp.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0317062
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASp regulatory sequences drive expression of a genomic fragment encoding the full-length Root coding region. The protein is tagged at the N-terminal end with EGFP.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Fails to rescue
Comments

The severe climbing defect of Root66 homozygous adults is completely rescued by expression of RootScer\UAS.P\T.T:Avic\GFP-EGFP under the control of either Scer\GAL4elav-C155 or Scer\GAL4insc-Mz1407. Expression under the control of either Scer\GAL4JO15, or simultaneous expression of both Scer\GAL4tilB.PK and Scer\GAL4nan.PK partially rescues the climbing defects, while expression under the control of Scer\GAL4wor.PA does not significantly rescue the mutant phenotype.

The lack of touch sensitivity seen in Root66 homozygous larvae is completely rescued by expression of RootScer\UAS.P\T.T:Avic\GFP-EGFP under the control of either Scer\GAL4elav-C155 or Scer\GAL4insc-Mz1407. Expression under the simultaneous control of both Scer\GAL4tilB.PK and Scer\GAL4nan.PK partially rescues the climbing defects, while expression under the control of Scer\GAL4wor.PA does not significantly rescue the mutant phenotype.

The reduced response of Root66 homozygous adults to sucrose in a proboscis extension reflex assay can be rescued by expression of RootScer\UAS.P\T.T:Avic\GFP-EGFP under the control of either Scer\GAL4elav-C155 or Scer\GAL4insc-Mz1407, with the rescue being stronger in females than in males.

Expression of RootScer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4elav-C155 partially restores a hearing response in Root66 homozygous adults. The ciliary rootlets of rescued chordotonal organs in both the femoral chordotonal organs and Johnston's organ are shorter than normal in these flies, while the rootlets of rescued external sensory organs appear normal in length.

Expression of RootScer\UAS.P\T.T:Avic\GFP-EGFP under the control of either Scer\GAL4elav-C155 or Scer\GAL4insc-Mz1407 completely restores fertility in Root66 homozygous males. Expression of RootScer\UAS.P\T.T:Avic\GFP-EGFP under the simultaneous control of both Scer\GAL4tilB.PK and Scer\GAL4nan.PK completely restores fertility in Root66 homozygous males.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
RootScer\UAS.P\T.T:Avic\GFP-EGFP
RootUASp.EGFP
Name Synonyms
Secondary FlyBase IDs
    References (1)