FB2025_01 , released February 20, 2025
Allele: Dmel\RootUASp.EGFP
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General Information
Symbol
Dmel\RootUASp.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0317062
Feature type
allele
Associated gene
Dmel\Root
Associated Insertion(s)
Carried in Construct
PBac{UASp-GFP-Root}
Key Links
Transgenic product class
genomic_DNA
(Chen et al., 2015)
wild_type
(Chen et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
genomic_DNA
(Chen et al., 2015)
wild_type
(Chen et al., 2015)
Mutagen
in vitro construct
(Chen et al., 2015)
Progenitor genotype
Carried in construct
PBac{UASp-GFP-Root}
Cytology
Description

UASp regulatory sequences drive expression of a genomic fragment encoding the full-length Root coding region. The protein is tagged at the N-terminal end with EGFP.

(Chen et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
Root
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4insc-Mz1407/RootUASp.EGFP rescues Root66

      (Chen et al., 2015)
      Partially rescues

      Scer\GAL4elav-C155/RootUASp.EGFP partially rescues Root66

      (Chen et al., 2015)

      Scer\GAL4JO15/RootUASp.EGFP partially rescues Root66

      (Chen et al., 2015)

      Scer\GAL4tilB.PK, Scer\GAL4nan.PK, RootUASp.EGFP partially rescues Root66

      (Chen et al., 2015)
      Fails to rescue

      Scer\GAL4wor.PA/RootUASp.EGFP fails to rescue Root66

      (Chen et al., 2015)
      Comments

      The severe climbing defect of Root66 homozygous adults is completely rescued by expression of RootScer\UAS.P\T.T:Avic\GFP-EGFP under the control of either Scer\GAL4elav-C155 or Scer\GAL4insc-Mz1407. Expression under the control of either Scer\GAL4JO15, or simultaneous expression of both Scer\GAL4tilB.PK and Scer\GAL4nan.PK partially rescues the climbing defects, while expression under the control of Scer\GAL4wor.PA does not significantly rescue the mutant phenotype.

      The lack of touch sensitivity seen in Root66 homozygous larvae is completely rescued by expression of RootScer\UAS.P\T.T:Avic\GFP-EGFP under the control of either Scer\GAL4elav-C155 or Scer\GAL4insc-Mz1407. Expression under the simultaneous control of both Scer\GAL4tilB.PK and Scer\GAL4nan.PK partially rescues the climbing defects, while expression under the control of Scer\GAL4wor.PA does not significantly rescue the mutant phenotype.

      The reduced response of Root66 homozygous adults to sucrose in a proboscis extension reflex assay can be rescued by expression of RootScer\UAS.P\T.T:Avic\GFP-EGFP under the control of either Scer\GAL4elav-C155 or Scer\GAL4insc-Mz1407, with the rescue being stronger in females than in males.

      Expression of RootScer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4elav-C155 partially restores a hearing response in Root66 homozygous adults. The ciliary rootlets of rescued chordotonal organs in both the femoral chordotonal organs and Johnston's organ are shorter than normal in these flies, while the rootlets of rescued external sensory organs appear normal in length.

      Expression of RootScer\UAS.P\T.T:Avic\GFP-EGFP under the control of either Scer\GAL4elav-C155 or Scer\GAL4insc-Mz1407 completely restores fertility in Root66 homozygous males. Expression of RootScer\UAS.P\T.T:Avic\GFP-EGFP under the simultaneous control of both Scer\GAL4tilB.PK and Scer\GAL4nan.PK completely restores fertility in Root66 homozygous males.

      (Chen et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      RootScer\UAS.P\T.T:Avic\GFP-EGFP
      RootUASp.EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)