FlyBase Allele Report: Dmel\ics[G4]

General Information

Symbol

Dmel\ics^G4

Species

D. melanogaster

Name

FlyBase ID

FBal0318750

Feature type

allele

Associated gene

Dmel\ics

Associated Insertion(s)

P{GawB}ics^G4

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{GawB}ics^G4

Cytology

Description

P{GawB} insertion into the ics gene.

(Ojelade et al., 2015)

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol dependence

CEA

(Ojelade et al., 2015)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical resistant | adult stage (with Df(2L)BSC147)

(Ojelade et al., 2015)

chemical resistant | adult stage | recessive

(Ojelade et al., 2015)

visible

(Ojelade et al., 2015)

Phenotype Manifest In

wing blade

(Ojelade et al., 2015)

Detailed Description

Statement

Reference

ics^G4 homozygotes display wing blisters. ics^G4/ics^G4 flies are more resistant to ethanol-induced sedation than ics^G4/+ or wild type flies, but have normal ethanol absorption and metabolism and show normal locomotion. ics^G4/Df(2L)BSC147 flies are more resistant to ethanol-induced sedation than Df(2L)BSC147/+ flies.

ics^G4/ics^G4 flies show a significant naive preference for ethanol, consuming more liquid food with ethanol on day 1 and 2 (and amounts consumed remain unchanged across 4 days) compared to controls (wild type flies gradually acquire a preference for ethanol over 3 days); total food consumption is not significantly different in mutants and controls.

(Ojelade et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ics^G4 has chemical resistant | adult stage phenotype, suppressible by Rac1^DN.UAS.cUa/Scer\GAL4^ics-G4

(Ojelade et al., 2015)

NOT suppressed by

Statement

Reference

ics^G4 has chemical resistant | adult stage phenotype, non-suppressible by mys^ts2/mys[+]

(Ojelade et al., 2015)

Suppressor of

Statement

Reference

ics^G4/ics^G4 is a suppressor of chemical sensitive | adult stage phenotype of mys^ts2

(Ojelade et al., 2015)

NOT Suppressor of

Statement

Reference

ics^G4/ics^G4 is a non-suppressor of chemical sensitive | adult stage phenotype of Rac1^DN.UAS.cUa, Scer\GAL4^ics-G4

(Ojelade et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

mys^ts2/+;ics^G4/ics^G4 flies have the same increased resistance to ethanol-induced sedation seen in ics^G4/ics^G4 mutants alone, unlike ethanol-sensitive mys^ts2/+ flies.

Scer\GAL4^ics-G4>Rac1^{DN.Scer\UAS.cUa};ics^G4/ics^G4 flies have the same increased sensitivity to ethanol-induced sedation seen in Scer\GAL4^ics-G4>Rac1^{DN.Scer\UAS.cUa} flies, unlike ethanol-resistant ics^G4/ics^G4 flies.

(Ojelade et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

ics^G4/Df(2L)BSC147 is rescued by Scer\GAL4^ics-G4/ics^UAS.cOa

(Ojelade et al., 2015)

ics^G4 is rescued by Scer\GAL4^ics-G4/ics^UAS.cOa

(Ojelade et al., 2015)

Partially rescued by

ics^G4 is partially rescued by Scer\GAL80^Mef2.mb247/Scer\GAL4^ics-G4/ics^UAS.cOa

(Ojelade et al., 2015)

Not rescued by

ics^G4 is not rescued by Scer\GAL4^ics-G4/Scer\GAL80^elav.PY/ics^UAS.cOa

(Ojelade et al., 2015)

Comments

Expression of ics^Scer\UAS.cOa driven by Scer\GAL4^ics-G4 rescues resistance to ethanol-induced sedation in ics^G4/ics^G4 (or ics^G4/Df(2L)BSC147) flies to control levels; there is no rescue when Scer\GAL80^elav.PY (to suppress neuronal ics expression) is also present. Expression of ics^Scer\UAS.cOa driven by Scer\GAL4^ics-G4 also restores ethanol consumption behavior in ics^G4/ics^G4 flies to wild type levels.

Expression of ics^Scer\UAS.cOa driven by Scer\GAL4^ics-G4 (using Scer\GAL80^ts.αTub84B to control timing of expression) during adulthood alone (but not throughout development) rescues resistance to ethanol-induced sedation in ics^G4/ics^G4 flies to control levels.

Expression of ics^Scer\UAS.cOa driven by Scer\GAL4^ics-G4 restores ethanol consumption behavior in ics^G4/ics^G4 flies to wild type levels; flies with additional presence of Scer\GAL80^Mef2.mb247 (to suppress ics expression in the mushroom bodies) show normal naive ethanol preference and normal resistance to ethanol-induced sedation, but unlike wild type do not acquire a preference for ethanol over 4 days.

(Ojelade et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Precise excision of the P{GawB} insertion reverts the mutant phenotype.

(Ojelade et al., 2015)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

ics^G4

(Ojelade et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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