Open Close
General Information
Symbol
Dmel\Arl2T30N.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0318766
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulates expression of Arl2 full-length coding sequence containing a T30N mutation, thus generating a dominant-negative form that mimics the constitutive GDP-bound form of Arl2.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Arl2T30N.Scer\UAS driven by Scer\GAL4insc.PU results in a significant increase in neuroblast number in the third instar larval central brain; introduction of heterozygous Arl2Δ156/+ further enhances neuroblast overgrowth at 72hr (and even further at 84hr) after larval hatching at 29[o]C. Arl2Δ156/+ larvae that also have expression of Arl2T30N.Scer\UAS driven by Scer\GAL4insc.PU have a slightly extended larval stage and more than half of metaphase neuroblasts have shorter misoriented spindles (compared to wild type), with a small percentage dividing orthogonally. Larvae with Arl2T30N.Scer\UAS overexpressed by Scer\GAL4insc.PU also have spindle misorientation in around 25% of neuroblasts, and around 13% of neuroblasts divide symmetrically. Unlike wild type telophase neuroblasts (where daughter cell sizes are distinct, with a NB/GMC diameter ratio of around 2), larvae with Arl2T30N.Scer\UAS overexpressed by Scer\GAL4insc.PU have a reduced ratio (around 1.65); introduction of Arl2Δ156/+ further reduces the ratio (around 1.5).

Arl2T30N.Scer\UAS clones have extra neuroblasts in both type I (Scer\GAL4ase.neuro) and II (Scer\GAL4wor.PA and Scer\GAL80ase.PN) neuroblast lineages, compared to a single neuroblast in control clones (third instar larval central brain).

After treatment on ice (to depolymerize microtubules and disrupt mitotic spindles), in larvae with Arl2T30N.Scer\UAS driven by Scer\GAL4insc.PU around 40% of metaphase neuroblasts reassembled less microtubule mass after 30 seconds recovery and only 46% form mitotic spindles (that are shorter and more narrow), compared to wild type.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of Arl2T30N.Scer\UAS increases severity of microtubule growth defects (shorter spindles, inability to form bipolar spindle) in the central brain of third instar larvae with expression of TBCDScer\UAS.cCa driven by Scer\GAL4insc.PU.

Co-expression of mspsFL.Scer\UAS dramatically reduces neuroblast number, largely restores spindle orientation in metaphase neuroblasts and rescues microtubule abnormalities (robust microtubule asters are present and spindle length is increased) in interphase neuroblasts in Arl2Δ156/+ larvae with Arl2T30N.Scer\UAS overexpressed by Scer\GAL4insc.PU.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Co-expression of Arl2WT.Scer\UAS rescues the overgrowth of neuroblasts seen in the central brain of third instar larvae with expression of Arl2T30N.Scer\UAS driven by Scer\GAL4insc.PU.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Arl2T30N.Scer\UAS
Arl2T30N.UAS
Name Synonyms
Secondary FlyBase IDs
    References (1)