FlyBase Allele Report: Dmel\Rtnl1[UAS.Tag:HA]

General Information

Symbol

Dmel\Rtnl1^UAS.Tag:HA

Species

D. melanogaster

Name

FlyBase ID

FBal0318878

Feature type

allele

Associated gene

Dmel\Rtnl1

Associated Insertion(s)

Carried in Construct

P{UAS-Rtnl1.HA}

Key Links

Transgenic product class

cDNA

(Summerville et al., 2016)

wild_type

(Summerville et al., 2016)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Summerville et al., 2016)

wild_type

(Summerville et al., 2016)

Mutagen

in vitro construct

(Summerville et al., 2016)

Progenitor genotype

Carried in construct

P{UAS-Rtnl1.HA}

Cytology

Description

UASt regulatory sequences drive expression of the Rtnl1-PB isoform (cDNA clone LD14068), which is tagged with Tag:HA.

(Summerville et al., 2016)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Rtnl1

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | third instar larval stage, with Scer\GAL4^Toll-6-D42

(Summerville et al., 2016)

Phenotype Manifest In

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^Toll-6-D42

(Summerville et al., 2016)

Detailed Description

Statement

Reference

Overexpression of Rtnl1^{Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^Toll-6-D42 leads to significant decreases in excitatory junction potential (EJP) amplitude (at 0.1 mM Ca[2+], accompanied by a significant decrease in frequency of synaptic transmission successes); corrected quantal content at 1.5 mM Ca[2+] does not significantly decrease compared to controls at the neuromuscular junction of third instar larvae.

(Summerville et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Rtnl1^UAS.Tag:HA/Scer\GAL4^Toll-6-D42 rescues Rtnl1¹⁸

(Pérez-Moreno et al., 2023)

Rtnl1^UAS.Tag:HA/Scer\GAL4^GMR94G06 rescues Rtnl1¹⁸

(Pérez-Moreno et al., 2023)

Rtnl1^UAS.Tag:HA/Scer\GAL4^da.G32 rescues Rtnl1^1.W

(Summerville et al., 2016)

Scer\GAL4^VGlut1-OK371, Scer\GAL4^Gli.PU, Rtnl1^UAS.Tag:HA, Scer\GAL4^Mef2.PR rescues Rtnl1^1.W

(Summerville et al., 2016)

Partially rescues

Rtnl1^UAS.Tag:HA/Scer\GAL4^arm.PS partially rescues Rtnl1^1.W

(Summerville et al., 2016)

Fails to rescue

Rtnl1^UAS.Tag:HA/Scer\GAL4^Mef2.PR fails to rescue Rtnl1^1.W

(Summerville et al., 2016)

Rtnl1^UAS.Tag:HA/Scer\GAL4^Toll-6-D42 fails to rescue Rtnl1^1.W

(Summerville et al., 2016)

Comments

Expression of Rtnl1^{Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^arm.PS significantly partially rescues decreased EJP amplitude at the NMJ of Rtnl1^1.W/Rtnl1^1.W third instar larvae; expression driven by Scer\GAL4^da.G32 fully rescues EJP amplitude (and frequency of synaptic successes). Expression of Rtnl1^{Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^Toll-6-D42 or Scer\GAL4^Mef2.PR fails to rescue decreases in EJP amplitude (and frequency of synaptic successes) at the NMJ of Rtnl1^1.W/Rtnl1^1.W third instar larvae. Expression of Rtnl1^{Scer\UAS.T:Ivir\HA1} driven simultaneously by Scer\GAL4^VGlut-OK371, Scer\GAL4^Mef2.PR and Scer\GAL4^Gli.PU rescues decreases in EJP amplitude (and frequency of synaptic successes) at the NMJ of Rtnl1^1.W/Rtnl1^1.W third instar larvae.