FlyBase Allele Report: Dmel\Urm1[n123]

General Information

Symbol

Dmel\Urm1ⁿ¹²³

Species

D. melanogaster

Name

FlyBase ID

FBal0319058

Feature type

allele

Associated gene

Dmel\Urm1

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Khoshnood et al., 2016)

Mutagen

P-element activity

(Khoshnood et al., 2016)

Progenitor genotype

P{SUPor-P}Urm1^KG08138

(Khoshnood et al., 2016)

Cytology

Description

620bp deletion removing the entire Urm1 locus. Generated by imprecise excision of P{SUPor-P}Urm1^KG08138.

(Khoshnood et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:7,639,819..7,640,438 [-]

Comment:

Inferred boundaries of a 620 bp deletion resulting from the imprecise excision of P{SUPor-P}Urm1^KG08138 which extends from the site of the insertion into the Urm1 coding sequence and removes the CDS.

(Khoshnood et al., 2016)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate

(Khoshnood et al., 2016)

abnormal oxidative stress response

(Khoshnood et al., 2016)

decreased body size

(Khoshnood et al., 2016)

decreased fecundity

(Khoshnood et al., 2016)

decreased rate of movement

(Khoshnood et al., 2016)

majority die during P-stage

(Khoshnood et al., 2016)

partially lethal - majority die

(Khoshnood et al., 2016)

short lived

(Khoshnood et al., 2016)

some die during embryonic stage

(Khoshnood et al., 2016)

Phenotype Manifest In

Detailed Description

Statement

Reference

20% of Urm1ⁿ¹²³/Urm1ⁿ¹²³ animals die during embryogenesis and 60% die at late pupal stages (compared to revertant controls); mutant escapers (primarily females, after a small but significant delay in development) have a significantly reduced lifespan, are smaller, less active, and have reduced fecundity compared to controls.

Urm1ⁿ¹²³/Urm1ⁿ¹²³ flies survive longer than controls under exposure to oxidative stress (10mM paraquat).

(Khoshnood et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Urm1ⁿ¹²³ has partially lethal - majority die phenotype, enhanceable by Df(2L)Exel7038

(Khoshnood et al., 2016)

Urm1ⁿ¹²³ has partially lethal - majority die phenotype, enhanceable by Df(2L)BSC215

(Khoshnood et al., 2016)

Suppressed by

Statement

Reference

Urm1ⁿ¹²³ has abnormal oxidative stress response phenotype, suppressible | partially by Prx2^KG05372/Prx2^KG05372

(Khoshnood et al., 2016)

Other

Statement

Reference

Prx5^EY02106, Urm1ⁿ¹²³ has abnormal oxidative stress response phenotype

(Khoshnood et al., 2016)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Df(2L)Exel7038/+ or Df(2L)BSC215/+ enhances lethality in Urm1ⁿ¹²³/Urm1ⁿ¹²³.

Prx5^EY02106/Prx5^EY02106 does not significantly alter survival rates of Urm1ⁿ¹²³/Urm1ⁿ¹²³ flies under oxidative stress, with double mutants surviving significantly longer than wild type or Prx5^EY02106/Prx5^EY02106 flies.

Jafrac1^KG05372/Jafrac1^KG05372 significantly suppresses the resistance to oxidative stress (increased survival under 10mM paraquat conditions) seen in Urm1ⁿ¹²³/Urm1ⁿ¹²³ flies.

(Khoshnood et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Urm1ⁿ¹²³ is rescued by Scer\GAL4^Act5C.PU/Urm1^UAS.cKa

(Khoshnood et al., 2016)

Urm1ⁿ¹²³ is rescued by Urm1^UAS.Tag:FLAG/Scer\GAL4^Act5C.PU

(Khoshnood et al., 2016)

Not rescued by

Urm1ⁿ¹²³ is not rescued by Scer\GAL4^Act5C.PU/Urm1^ΔGG.UAS