FlyBase Allele Report: Scer\GAL4[DBD.R71G01]

General Information

Symbol

Scer\GAL4^DBD.R71G01

Species

S. cerevisiae

Name

FlyBase ID

FBal0319144

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{R71G01-GAL4.DBD}

PBac{R71G01-GAL4.DBD}

Also Known As

R71G01-DBD, P1-Gal4 DBD, R71G01-GAL4.DBD

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Rubin Laboratory at Janelia Farm, 2017, Inagaki et al., 2014)

Progenitor genotype

Carried in construct

P{R71G01-GAL4.DBD}

PBac{R71G01-GAL4.DBD}

Cytology

Description

The GMR71G01 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).

(Rubin Laboratory at Janelia Farm, 2017)

Allele components

Component

Use(s)

Regulatory region(s)

GMR71G01

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female

adult lateral horn | restricted

Comment: when combined with P{R15A01-p65.AD}

(Hoopfer et al., 2015)

adult stage | male

adult fruitless P1 (male) neuron | subset

Comment: when combined with P{R15A01-p65.AD}

(Hoopfer et al., 2015)

adult lateral horn | restricted

Comment: when combined with P{R15A01-p65.AD}

(Hoopfer et al., 2015)

adult stage

adult fruitless P1 (male) neuron | subset

Comment: when combined with P{R15A01-p65.AD} (combination referred to as 'P1-splitGAL4')

when combined with P{R15A01-p65.AD} (combination referred to as 'P1a')

when combined with P{R17D06-p65.AD} (combination referred to as 'P1c')

(Wu et al., 2019, Zhang et al., 2018)

adult bridle subesophageal neuron

Comment: when combined with P{VT026953-p65.AD} (combination referred to as 'SS32066')

(Sterne et al., 2021)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | adult stage, with Hsap\RELA^AD.R15A01, dsx^TH13361.N

(Han et al., 2022)

abnormal behavior | adult stage, with Hsap\RELA^AD.R15A01, dsx^{UAS.M.Tag:MYC}

(Han et al., 2022)

abnormal behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Hsap\RELA^AD.R15A01

(Hoopfer et al., 2015)

abnormal behavior | adult stage | conditional, with Crei\ChR1::Vcar\ChR1::Vcar\ChR2^{ReaChR.UAS.Citrine}, Hsap\RELA^AD.R15A01

(Hoopfer et al., 2015)

abnormal behavior | adult stage | heat sensitive, with Hsap\RELA^AD.R15A01, TrpA1^UAS.(B).cKa

(Hoopfer et al., 2015)

abnormal courtship behavior, with Hsap\RELA^AD.R15A01, Rdl^JF01227

(Kallman et al., 2015)

abnormal courtship behavior | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Hsap\RELA^AD.R15A01

(Hoopfer et al., 2015)

abnormal courtship behavior | conditional, with Crei\ChR1::Vcar\ChR1::Vcar\ChR2^{ReaChR.UAS.Citrine}, Hsap\RELA^AD.R15A01

(Hoopfer et al., 2015)

abnormal courtship behavior | heat sensitive, with Hsap\RELA^AD.R15A01, TrpA1^UAS.(B).cKa

(Hoopfer et al., 2015)

abnormal courtship behavior | male limited | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Hsap\RELA^AD.R15A01

(Kallman et al., 2015)

abnormal locomotor behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Hsap\RELA^AD.R15A01

(Hoopfer et al., 2015)

abnormal locomotor behavior | adult stage | conditional, with Crei\ChR1::Vcar\ChR1::Vcar\ChR2^{ReaChR.UAS.Citrine}, Hsap\RELA^AD.R15A01

(Hoopfer et al., 2015)

abnormal neuroanatomy | adult stage, with Hsap\RELA^AD.R15A01, dsx^TH13361.N, dsx^{UAS.F.Tag:FLAG}

(Han et al., 2022)

decreased cell number | adult stage, with Hsap\RELA^AD.R15A01, dsx^TH13361.N, dsx^{UAS.F.Tag:FLAG}

(Han et al., 2022)

decreased rate of copulation, with Hsap\RELA^AD.R15A01, dsx^TH13361.N

(Han et al., 2022)

decreased rate of courtship behavior, with Hsap\RELA^AD.R15A01, dsx^TH13361.N

(Han et al., 2022)

Phenotype Manifest In

adult fruitless P1 (male) neuron | decreased number, with Hsap\RELA^AD.R15A01, dsx^TH13361.N, dsx^{UAS.F.Tag:FLAG}

(Han et al., 2022)

Detailed Description

Statement

Reference

Brief activation of Scer\GAL4^AD.R15A01 and Scer\GAL4^DBD.R71G01 neurons leads to male flies displaying persistent courtship behavior (tracking and singing) towards a rotating magnet that was previously of no interest.

(Clowney et al., 2015)

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 29[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R71G01 and Hsap\RELA^AD.R15A01 in adult males significantly increases aggressive lunging behavior and wing extensions towards other males, compared to controls.

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with 685 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R71G01 and Hsap\RELA^AD.R15A01 in adult males significantly increases wing extensions towards other males during illumination and significantly increases aggressive lunging behavior towards other males following illumination, compared to controls; locomotor velocity is also significantly reduced during illumination, compared to baseline; activation in solitary males results in a sustained increase in wing extensions during and after illumination, compared to controls.

Optogenetic activation (using Crei\ChR1::Vcar\ChR1::Vcar\ChR2^{ReaChR.UAS.Citrine} with 530 nm (green) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R71G01 and Hsap\RELA^AD.R15A01 in adult males significantly increases wing extensions towards other males during illumination and significantly increases aggressive lunging behavior towards other males for at least 10 min following illumination, compared to controls; activation in solitary males results in an increase in wing extensions during and up to 5 min after illumination, compared to controls; for at least 10 min after activation, a solitary male that is introduced to another male will significantly increase aggressive lunging behavior, compared to controls; locomotor velocity is significantly reduced during and up to 1 min following illumination, compared to controls.

(Hoopfer et al., 2015)

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with bright white light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R71G01 and Hsap\RELA^AD.R15A01 in solitary adult males significantly increases wing extensions, compared to baseline; this increase is significantly reduced when Scer\GAL4^GMR43D01 cells are simultaneously activated.

(Kallman et al., 2015)

Activation of Scer\GAL4^AD.R15A01 and Scer\GAL4^DBD.R71G01 neurons triggers wing extension behavior; depending on the combination of UAS and light used, different numbers of flies show this behavior: 0% with blue light activation of Crei\ChR2^Scer\UAS.cSa, Crei\ChR2^{H134R.Scer\UAS.2A.T:Avic\GFP-EYFP} or Crei\ChR2^{C128T.Scer\UAS.T:Avic\GFP-YFP}; 0% with red or green light activating Crei\ChR1[C1V1.T-T.Scer\UAS. T:Avic \GFP-EYFP]; 40% (red) or 100% (green) light activating Crei\ChR1^{ReaChR.Scer\UAS.T:Crei\ChR2,T:Avic\GFP-mCitrine}. Social isolation lowers the threshold for Crei\ChR1^{ReaChR.Scer\UAS.T:Crei\ChR2,T:Avic\GFP-mCitrine}-activated male courtship behavior.