FB2025_01 , released February 20, 2025
Allele: Dmel\pimdk.UASp.EGFP
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General Information
Symbol
Dmel\pimdk.UASp.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0319199
Feature type
allele
Associated gene
Dmel\pim
Associated Insertion(s)
Carried in Construct
P{UASp-GFP-pim.dk}
Key Links
Transgenic product class
missense_variant
(Guo et al., 2016)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Guo et al., 2016)
Mutagen
in vitro construct
(Guo et al., 2016)
Progenitor genotype
Carried in construct
P{UASp-GFP-pim.dk}
Cytology
Description

UASp regulatory sequences drive expression of a stabilised form of pim which lacks both the D-box and KEN-box. The protein is tagged with at the N-terminal end with EGFP.

(Guo et al., 2016)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
pim
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Eggs with expression of pimdk.Scer\UAS.P\T.T:Avic\GFP-EGFP driven in the female germline by Scer\GAL4mat.αTub67C.T:Hsim\VP16 fail to hatch but the majority appear to complete meiosis (89% of eggs contain a polar body and 88% of polar bodies contain three X-chromosomes); in wild type eggs 96% complete meiosis and contain a single or, rarely, two polar bodies: 90% of polar bodies contain three X-chromosomes. Zygotic development is arrested early and most embryos contain only 1-2 zygotic nuclei that range in appearance from normal to aberrant. Scer\GAL4mat.αTub67C.T:Hsim\VP16>pimdk.Scer\UAS.P\T.T:Avic\GFP-EGFP embryos contain several free centrosomes and a single aberrant mitotic spindle with re-replicated DNA. Wild type embryos have up to 6 X-arm FISH signals in their polar bodies (depending on age), whereas mutants almost always have 3 (failure of polar body chromosome arm segregation and chromosome segregation in syncytial mitosis).

      Unlike pimdsRNA.5'.Scer\UAS.P\T or pimdk.Scer\UAS.P\T.T:Avic\GFP-EGFP expression alone (permit some embryonic development), co-expression driven by Scer\GAL4mat.αTub67C.T:Hsim\VP16 leads to arrested development in the first (or rarely the second) mitotic division in embryos; in 50% of embryos, the lone mitotic spindle has a single X-cent FISH signal and in 50% there is no associated X-cent signal - indicating the embryo forms solely from the male pronucleus; the majority of embryos with co-expression contain polar bodies (72%) and contain 4 X-chromosomes, and polar body arms fail to lose arm cohesion. Co-expression of pimdsRNA.5'.Scer\UAS.P\T and pimScer\UAS.P\T.T:Avic\GFP-EGFP driven by Scer\GAL4mat.αTub67C.T:Hsim\VP16 leads to similar phenotypes as seen with expression pimdsRNA.5'.Scer\UAS.P\T or pimdk.Scer\UAS.P\T.T:Avic\GFP-EGFP alone. pimdsRNA.5'.Scer\UAS.P\T and pimdk.Scer\UAS.P\T.T:Avic\GFP-EGFP co-expression driven by Scer\GAL4mat.αTub67C.T:Hsim\VP16 leads to delays in cohesion release and consequent chromosome movements in meiosis and in the majority (79%) of eggs, centromeric cohesion persists into post-meiotic interphase; centromeric cohesion persists in 34% and arm cohesion persists in 22% of polar bodies.

      (Guo et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      pimdk.Scer\UAS.P\T.T:Avic\GFP-EGFP
      pimdk.UASp.EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)