Scer\GAL4mat.αTub67C.T:Hsim\VP16>pimdsRNA.5'.Scer\UAS.P\T oocytes do not undergo premature chromosome segregation, eggs appear to complete meiosis and make a polar body in which chromosome arms fail to lose cohesion over time (similar to pimdk.Scer\UAS.P\T.T:Avic\GFP-EGFP). Embryos also display mitotic defects (milder than as seen with pimdk.Scer\UAS.P\T.T:Avic\GFP-EGFP).
Unlike pimdsRNA.5'.Scer\UAS.P\T or pimdk.Scer\UAS.P\T.T:Avic\GFP-EGFP expression alone (permit some embryonic development), co-expression driven by Scer\GAL4mat.αTub67C.T:Hsim\VP16 leads to arrested development in the first (or rarely the second) mitotic division in embryos; in 50% of embryos, the lone mitotic spindle has a single X-cent FISH signal and in 50% there is no associated X-cent signal - indicating the embryo forms solely from the male pronucleus; the majority of embryos with co-expression contain polar bodies (72%) and contain 4 X-chromosomes, and polar body arms fail to lose arm cohesion. Co-expression of pimdsRNA.5'.Scer\UAS.P\T and pimScer\UAS.P\T.T:Avic\GFP-EGFP driven by Scer\GAL4mat.αTub67C.T:Hsim\VP16 leads to similar phenotypes as seen with expression pimdsRNA.5'.Scer\UAS.P\T or pimdk.Scer\UAS.P\T.T:Avic\GFP-EGFP alone. pimdsRNA.5'.Scer\UAS.P\T and pimdk.Scer\UAS.P\T.T:Avic\GFP-EGFP co-expression driven by Scer\GAL4mat.αTub67C.T:Hsim\VP16 leads to delays in cohesion release and consequent chromosome movements in meiosis and in the majority (79%) of eggs, centromeric cohesion persists into post-meiotic interphase; centromeric cohesion persists in 34% and arm cohesion persists in 22% of polar bodies.
