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General Information
Symbol
Dmel\shvUAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0319441
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-shv
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of shv tagged with Tag:HA.

UAS regulatory sequences drive expression of the full-length shv coding region.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of shvScer\UAS.T:Ivir\HA1 under the control of either Scer\GAL4nSyb.PS or Scer\GAL4how-24B does not perturb synaptic growth as the number of boutons as well as immature, undifferentiated 'ghost boutons' on the neuromuscular junctions in third instar larvae is comparable to controls. Scer\GAL4nSyb.PS-driven expression of shvScer\UAS.T:Ivir\HA1 also has no significant effect on the amplitude of either miniature or evoked excitatory postsynaptic potentials.

Expression of shvScer\UAS.cLa driven in hub and cyst cells by Scer\GAL4C833 does not significantly affect hub cell number (up to 35 days old) but does significantly decrease germline stem cell number (at 3, 7, 14, 35 and 50 days post eclosion) in the testes compared to controls. Expression of shvScer\UAS.cLa driven in hub cells by Scer\GAL4upd1.PU does not significantly affect hub cell number (up to 35 days old) in the testes compared to controls. Expression of shvScer\UAS.cLa driven in germ cells by Scer\GAL4nos.UTR.T:Hsim\VP16 does not significantly affect the number of germline stem cells per testes (up to 35 days old) but does significantly increase the number of hub cells at 3 and 50 days post eclosion (but not 7, 14 or 35 days) compared to controls.

Expression of shvScer\UAS.cLa driven by Scer\GAL4C833, Scer\GAL4nos.UTR.T:Hsim\VP16 or Scer\GAL4upd1.PU increases hub cell number per testes in 50 day old flies compared to controls. Expression of shvScer\UAS.cLa driven by Scer\GAL4upd1.PU increases germline stem cell number per testes in 50 day old flies compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
NOT Suppressor of
Phenotype Manifest In
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Expression of shvScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4nSyb.PS (but not Scer\GAL4how-24B) rescues the increased number of boutons on neuromuscular junctions characteristic for mysts1 mutant third instar larvae.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescues
Comments

Expression of shvScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4nSyb.PS (but not Scer\GAL4how-24B) rescues the increased number of boutons on neuromuscular junctions as well as the decreased miniature excitatory postsynaptic potential amplitude observed in shv1 mutant third instar larvae.

Expression of shvScer\UAS.cLa driven in hub and cyst cells by Scer\GAL4C833 rescues hub integrity and germline stem cell number (at all ages tested), along with rescuing hub mislocalization, in shv1/shv1 testes. Expression of shvScer\UAS.cLa driven by Scer\GAL4C587 rescues hub integrity and germline stem cell number in shv1/shv1 testes. Expression of shvScer\UAS.cLa driven in hub cells by Scer\GAL4upd1.PU fails to rescue and indeed exacerbates hub integrity in shv1/shv1 testes (at all ages tested). Expression of shvScer\UAS.cLa driven in germ cells by Scer\GAL4nos.UTR.T:Hsim\VP16 partially preserves the hub (at all ages) and protects against decreases in germline stem cell number (except by day 35) in shv1/shv1 testes.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
shvScer\UAS.T:Ivir\HA1
shvScer\UAS.cLa
shvUAS.Tag:HA
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Lee
Secondary FlyBase IDs
    References (2)