UASt regulatory sequences drive expression of DsRed2 coding sequences, with a CTG sequence repeated an uninterrupted 130 times in the 3'UTR.
eye color defective, with Scer\GAL4GMR.PF
increased mortality during development, with Zzzz\CAG100.UAS.DsRed2, Scer\GAL4how-24B
viable, with Scer\GAL4da.G32
viable, with Scer\GAL4elav-C155
viable, with Scer\GAL4how-24B
eye, with Scer\GAL4GMR.PF
retina, with Scer\GAL4GMR.PF
Expression of Disc\RFP(CTG)130.Scer\UAS in the muscle (Scer\GAL4how-24B), nervous system (Scer\GAL4elav-C155) or ubiquitously (Scer\GAL4da.G32) does not affect viability.
Expression of Disc\RFP(CTG)130.Scer\UAS in the developing eye, under the control of Scer\GAL4GMR.PF gives a mild rough eye phenotype, with abnormal eye pigmentation and disruption of retinal integrity.
Co-expression of Disc\RFPCAG100.DsRed2.Scer\UAS with Disc\RFP(CTG)130.Scer\UAS in the muscle, under the control of Scer\GAL4how-24B, results in developmental lethality.
Expression of Disc\RFP(CTG)130.Scer\UAS in the muscle, under the control of Scer\GAL4how-24B, does not generate a phenotype.
Zzzz\CTG130.UAS.DsRed2/Disc\RFP(CTG)130.Scer\UAS, Zzzz\CAG100.UAS.DsRed2/Disc\RFPCAG100.DsRed2.Scer\UAS, Scer\GAL4how-24B has increased mortality during development phenotype, suppressible by Dcr-2L811fsX
A Dcr-2L811fsX homozygous mutant background suppresses the developmental lethality associated with expression of Disc\RFP(CTG)130.Scer\UAS and Disc\RFPCAG100.DsRed2.Scer\UAS in the developing muscle (under the control of Scer\GAL4how-24B)