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General Information
Symbol
Dmel\pcm14
Species
D. melanogaster
Name
FlyBase ID
FBal0319758
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:
Inferred boundaries of a 3,501 bp deletion resulting from the imprecise excision of P{EP}EP1526. The deletion extends towards pcm from the insertion site and removes 3,068 bp from the 3' end of pcm as well as CR34260.
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
A 3,501 bp deletion extending from the progenitor insertion site towards pcm, deleting 3,068 bp into the 3' of the gene, completely removing exons 7-11 and part of exon 6. The 5' of the neighbouring non-coding RNA CR43260 is also deleted.
Caused by aberration
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Apoptosis is seen in the wing pouch area of homozygous third larval instar wing imaginal discs (no cell death is seen in wild-type wing discs at the same stage).
pcm14 mutant third instar larvae have significantly smaller wings discs compared to controls and are developmentally delayed.
pcm14 homozygous females and hemizygous males all die during pupation before any adult structures are formed although their growth rate and size during larval stages is unaffected. Onset of pupariation however is significantly delayed by around 32 hours compared to wild-type, the larvae continue to feed during this period and are thus significantly heavier just before pupariation than controls. pcm14/pcm5 transheterozygote adults cannot emerge from their pupal cases and remains stuck. The same phenotype is seen when pcm5 is combined with Df(1)ED7452 deficiency, suggesting that pcm14 is genetically a null allele. pcm14 homozygote third instar larvae have significantly smaller wing, metathoracic leg, haltere as well as eye imaginal discs compared to wild-type controls. The wing discs are also delayed both in growth and differentiation (assessed by wg pattern) and even though the entry into pupal stage is delayed in pcm14 homozygotes, their wing discs just before the onset of pupariation are still significantly smaller compared to controls. pcm14/pcm14 third instar larval wing discs display significantly increased level of apoptosis (assessed by Caspase-3 staining). However, both the mitotic and the S-phase index (calculated as number of cells per area) are also increased compared to wild-type, suggesting the wing disc cells are dividing more to counteract the cell loss due to apoptosis. Somatic clones of pcm14/pcm14 cells in the third instar larval wing discs are much smaller compared to their wild-type twin spot counterparts.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Statement
Reference
Suppressor of
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
The reduced wing disc size characteristic for pcm14 mutant third instar larvae is fully rescued by expression of Dis3l2GD9240 under the control of Scer\GAL469B in the mutant background but the larvae are still developmentally delayed.
The dramatic increase in apoptosis (assessed by Caspase-3 staining) in the third instar larval wing disc characteristic for pcm14 homozygote mutants is strongly suppressed by combination with Df(3L)H99 in heterozygote state, the reduced size of the disc is partially restored and so is its differentiation (the disturbed wg pattern is recovered). The reduced size of the third larval instar imaginal wing disc characteristic for pcm14 homozygotes is strongly rescued by combination with heterozygous Df(3L)H99 deficiency, homozygous Dark82 allele or by expression of Diap1Scer\UAS.cHa under the control of Scer\GAL469B (using tub-Gal80[ts] to limit the time of expression) in the mutant background. The resulting disc size is intermediate between that of pcm14 alone and that of Df(3L)H99/+ or Dark82/Dark82 or Diap1Scer\UAS.cHa-expressing discs (each of which alone gives rise to wing disc bigger than wild-type) and smaller than wild-type.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Fails to complement
Partially rescued by
Not rescued by
Comments
The pupal lethality of pcm14 mutants is fully rescued by expression of pcmScer\UAS.cWa under the control of the Scer\GAL469B but not that of the Scer\GAL4nub-AC-62 driver. The reduced size of third instar larval wing discs characteristic for pcm14 homozygote larvae is fully rescued by expression of pcmScer\UAS.cWa when driven by the Scer\GAL469B and partially rescued when controlled by the Scer\GAL4nub-AC-62 driver. Neither the pupal lethality of pcm14 mutants nor the reduced size of their third instar larval wing discs is rescued by expression of pcmScer\UAS.ND under the control of Scer\GAL469B.
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (5)