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General Information
Symbol
Dmel\Nprl31
Species
D. melanogaster
Name
FlyBase ID
FBal0319815
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Mutagen
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:

Approximate boundaries of a deletion that extends from amino acid 61-559 of Nprl3. The deletion causes a frameshift which leads to translation termination after 5 amino acids.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Deletion that removes most of the Nprl3 coding region. Sequence encoding amino acids 61-559 is deleted, and a frameshift is generated, resulting in a new stop codon after 5 amino acid residues.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

A high percentage of Nprl31/Nprl31 females die as pupae or pharate adults.

Nprl31 as well as Nprl31/Df(3L)ED4515 mutants are partially lethal (majority die at the pupae/pharate adult stage) but the Nprl31/Df(3L)ED4515 transheterozygotes eclose at significantly higher rate (though majority die as pharate adults) than the Nprl31 homozygotes - likely due to additional negative impact of their genetic background. Nprl31/Df(3L)ED4515 adults show reduced climbing ability.

The size of cells in the Nprl31 somatic follicle cell clones is significantly increased compared to adjacent heterozygous cells and the egg chambers containing Nprl31 mutant germline clones are also increased in size.

Nprl31 mutants - Nprl31/Df(3L)ED4515 transheterozygotes or homozygotes - display reduced survival rates under nutrient-liming conditions (complete starvation or amino acid starvation, respectively) and fail to activate autophagy (assessed by LysoTracker staining in larval fat body) upon starvation.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT Enhancer of
Statement
Reference
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Fat bodies from Wdr241/Wdr241;Nprl31/Nprl31 third instar larvae accumulate large numbers of late endosomes or lysosomes (not autolysosomes); these are not observed in single Nprl31/Nprl31 mutants, and autolysosomes in Wdr241/Wdr241 single mutants are larger (and activate autophagy) rather than the than puncta in the double mutants (do not activate autophagy).

The partial lethality of either Nprl21 or Nprl31 single mutants is not increased further in the Nprl21;Nprl31 double mutants but can be significantly suppressed by combination with a single copy of TorA948V.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The partial lethality as well as reduced survival rates and failure to activate autophagy (assessed by LysoTracker staining in larval fat body) under nutrient-limiting conditions characteristic for Nprl31 mutants (Nprl31 homozygotes or Nprl31/Df(3L)ED4515 transheterozygotes) is rescued by expression of Nprl3Scer\UAS.P\T.T:Zzzz\FLAG,T:Ivir\HA1 under the control of Scer\GAL4da.G32. Expression under the Scer\GAL4Cg.PA driver also rescues the increased lethality and climbing defects of Nprl31/Df(3L)ED4515 animals and the latter is partially suppressed also by Scer\GAL4G14 and Scer\GAL4elav.PLu-driven expression of Nprl3Scer\UAS.P\T.T:Zzzz\FLAG,T:Ivir\HA1.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (3)