Open Close
General Information
Symbol
Dmel\U2A1.W
Species
D. melanogaster
Name
FlyBase ID
FBal0319834
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    deletion
    Comment:
    A 7 bp deletion in the U2A coding sequence within the leucine-rich repeat causes a frameshift.
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference
    7bp deletion in exon 1 (nucleotides 253-259 relative to the start codon are removed). This results in a frameshift and loss of the conserved leucine-rich repeat C-terminal and U2A domains.
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference
    Using U2Ahs.PW in combination with heat shock to rescue lethality (then switching off rescue during adulthood to study mutant phenotypes), the following phenotypes were observed in U2A1.W/U2A1.W flies: males are sterile and lack mature sperm, the apical region of the testes contains many germ cells resembling early germ cells (likely spermatogonial cells) but frequently lack differentiating germ cell cysts. Mutants have a small population of germline stem cells around the hub, similar to wild type. Mutant testes contain many cysts with more than 16 spermatogonia and only 25% of cysts progress to the spermatocyte state (many of which have over 32 spermatocytes per cyst) and many germ cell cysts are mitotic, suggesting excessive proliferation of spermatogonia that fail to enter meiosis and differentiation.
    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Suppressed by
    Statement
    Reference
    Phenotype Manifest In
    Additional Comments
    Genetic Interactions
    Statement
    Reference
    Xenogenetic Interactions
    Statement
    Reference
    Expression of Hsap\SNRPA1Scer\UAS.P\T.cWa driven by Scer\GAL4αTub84B.PL suppresses lethality in U2A1.W/U2A1.W mutants: flies are fertile and have normal testis morphology and spermatogonial development.
    Complementation and Rescue Data
    Comments
    Continuous heat shock treatment with U2Ahs.PW rescues lethality of U2A1.W/U2A1.W, allowing survival to adulthood. Expression of U2AScer\UAS.P\T.cWa driven by Scer\GAL4αTub84B.PL rescues sterility in U2A1.W/U2A1.W males.
    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (2)
    Reported As
    Symbol Synonym
    U2A1.W
    Name Synonyms
    Secondary FlyBase IDs
      References (1)