FlyBase Allele Report: Dmel\U2A[RNAi.UASp]

General Information

Symbol

Dmel\U2A^RNAi.UASp

Species

D. melanogaster

Name

FlyBase ID

FBal0319837

Feature type

allele

Associated gene

Dmel\U2A

Associated Insertion(s)

Carried in Construct

P{UASp-U2A.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Wu et al., 2016)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Wu et al., 2016)

Mutagen

in vitro construct

(Wu et al., 2016)

Progenitor genotype

Carried in construct

P{UASp-U2A.RNAi}

Cytology

Description

UASp regulatory sequences drive expression of a hairpin RNA targeting U2A.

(Wu et al., 2016)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

U2A

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of azoospermia

CEA

(Wu et al., 2016)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

male semi-fertile, with Scer\GAL4^{VP16.nanos.UTR}

(Wu et al., 2016)

male sterile, with Scer\GAL4^VP16.bam

(Wu et al., 2016)

male sterile, with Scer\GAL4^αTub84B.PL

(Wu et al., 2016)

Phenotype Manifest In

spermatogonium, with Scer\GAL4^VP16.bam

(Wu et al., 2016)

spermatozoon, with Scer\GAL4^VP16.bam

(Wu et al., 2016)

Detailed Description

Statement

Reference

Flies with expression of U2A^{dsRNA.shRNA.Scer\UAS.P\T} driven by Scer\GAL4^αTub84B.PL only during adulthood (with TubGAL80ts put at 29[o]C after eclosion for 7 days) leads to complete male sterility. Expression of U2A^{dsRNA.shRNA.Scer\UAS.P\T} driven by Scer\GAL4^{nos.UTR.T:Hsim\VP16} leads to reduced fertility in males (but not females); expression driven by Scer\GAL4^C587 (in adulthood with TubGAL80ts) or Scer\GAL4^upd1-E132 has no effect on fertility, whereas expression driven by Scer\GAL4^{bam.T:Hsim\VP16} leads to complete male sterility and defects in spermatogonial differentiation, with absence of mature sperm.

(Wu et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^VP16.bam, U2A^RNAi.UASp has male sterile phenotype, suppressible by Hsap\SNRPA1^UASp.cWa, Scer\GAL4^VP16.bam

(Wu et al., 2016)

NOT suppressed by

Statement

Reference

Scer\GAL4^VP16.bam, U2A^RNAi.UASp has male sterile phenotype, non-suppressible by Hsap\SNRPA1^Q148R.UASp, Scer\GAL4^VP16.bam

(Wu et al., 2016)

Suppressor of

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}/U2A^RNAi.UASp is a suppressor of decreased cell number phenotype of Scer\GAL4^{VP16.nanos.UTR}, mei-P26^UASp.gs

(Wu et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^VP16.bam, U2A^RNAi.UASp has spermatogonium phenotype, suppressible by Hsap\SNRPA1^UASp.cWa, Scer\GAL4^VP16.bam

(Wu et al., 2016)

Scer\GAL4^VP16.bam, U2A^RNAi.UASp has spermatozoon phenotype, suppressible by Hsap\SNRPA1^UASp.cWa, Scer\GAL4^VP16.bam

(Wu et al., 2016)

NOT suppressed by

Statement

Reference

Scer\GAL4^VP16.bam, U2A^RNAi.UASp has spermatogonium phenotype, non-suppressible by Hsap\SNRPA1^Q148R.UASp, Scer\GAL4^VP16.bam

(Wu et al., 2016)

Scer\GAL4^VP16.bam, U2A^RNAi.UASp has spermatozoon phenotype, non-suppressible by Hsap\SNRPA1^Q148R.UASp, Scer\GAL4^VP16.bam

(Wu et al., 2016)

Suppressor of

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}/U2A^RNAi.UASp is a suppressor of male germline cell phenotype of Scer\GAL4^{VP16.nanos.UTR}, mei-P26^UASp.gs

(Wu et al., 2016)

Other

Statement

Reference

Scer\GAL4^VP16.bam, U2A^RNAi.UASp, mei-P26^RNAi.UASp has spermatocyte phenotype

(Wu et al., 2016)

Scer\GAL4^VP16.bam, U2A^RNAi.UASp, mei-P26^RNAi.UASp has spermatogonial cyst phenotype

(Wu et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of U2A^{dsRNA.shRNA.Scer\UAS.P\T} suppresses germ cell loss in testes with expression of mei-P26^{Scer\UAS.P\T.gs} driven by Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

Simultaneous knockdown of mei-P26^{dsRNA.shRNA.Scer\UAS.P\T} and U2A^{dsRNA.shRNA.Scer\UAS.P\T} driven by Scer\GAL4^{bam.T:Hsim\VP16} causes a more severe phenotype compared with individual knockdown, with increased accumulation of spermatogonial cysts and fewer spermatocytes in the apical region of the testes.

(Wu et al., 2016)

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\SNRPA1^{Scer\UAS.P\T.cWa} restores spermatogenesis, fertility and normal spermatogonial differentiation and presence of mature sperm in testes with expression of U2A^{dsRNA.shRNA.Scer\UAS.P\T} driven by Scer\GAL4^{bam.T:Hsim\VP16}. Co-expression of Hsap\SNRPA1^{Q148R.Scer\UAS.P\T} does not rescue fertility or spermatogonial maturation defects in flies with expression of U2A^{dsRNA.shRNA.Scer\UAS.P\T} driven by Scer\GAL4^{bam.T:Hsim\VP16}.

(Wu et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

U2A^RNAi.UASp

U2A^{dsRNA.shRNA.Scer\UAS.P\T}

U2A^{dsRNA.shRNA.UASp}

Name Synonyms

Secondary FlyBase IDs

References (2)

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