FB2025_01 , released February 20, 2025
Allele: Dmel\Dis31
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General Information
Symbol
Dmel\Dis31
Species
D. melanogaster
Name
FlyBase ID
FBal0319956
Feature type
allele
Associated gene
Dmel\Dis3
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Snee et al., 2016)
Mutagen
ethyl methanesulfonate
(Snee et al., 2016)
Progenitor genotype
Cytology
Description

Amino acid replacement: Q252term.

(Snee et al., 2016)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:24,334,298..24,334,298 [+]
Nucleotide change:

C24334298T

Reported nucleotide change:

C866T

Amino acid change:

Q252term | Dis3-PA; Q252term | Dis3-PB

Reported amino acid change:

Q252term

(Snee et al., 2016)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  multiple myeloma
      CEA
      (Snee et al., 2016)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Dis31/Dis31 mutants survive at the second instar larval stage for an extended period and then die. Dis31/Dis31 wing disc clones in second instar larvae have significantly fewer cells per clone compared to controls and mutant cells undergo only one cell division in a 48hr time window (compared to 4 in wild type cells).

      Dis31/Dis32 third instar larvae with expression of Dis3S786E.Scer\UAS.P\T driven by Scer\GAL4tub.PU lack imaginal discs, have reduced brain and ventral nerve cord size, whereas other tissues that do not grow via mitotic cell cycles (salivary glands) are generally normal in size and morphology; larval neuroblasts have multipolar spindles; larval neuroblasts have multipolar, disorganized or short spindles, 5% of mitotic neuroblasts display aneuploidy, 44% display over condensed chromosomes and more neuroblasts are mitotically active than controls.

      Dis31/Dis32 larvae have shortened mitotic spindles in neuroblasts.

      Dis3G5039/Dis31 flies are viable, largely infertile and morphologically normal (apart from small bristles and occasional crumpled wings); a large percentage of stage 5-6 egg chambers show over proliferation, with excess posterior follicle cells.

      (Snee et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      Dis31/Dis3[+] is a suppressor of chemical sensitive | adult stage phenotype of Otud6C183A

      (Villa et al., 2024)

      Dis31/Dis3[+] is a suppressor of increased mortality | adult stage phenotype of Otud6C183A

      (Villa et al., 2024)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Dis31 is rescued by Dis3UASp.WT/Scer\GAL4Tub.PU

      (Snee et al., 2016)

      Dis31 is rescued by Dis3S786A.UASp/Scer\GAL4Tub.PU

      (Snee et al., 2016)

      Dis31/Dis3G5039 is rescued by Scer\GAL4Act.PU/Dis3G5039

      (Snee et al., 2016)
      Partially rescued by

      Dis31/Dis32 is partially rescued by Dis3S786E.UASp/Scer\GAL4Tub.PU

      (Snee et al., 2016)
      Comments

      Expression of Dis3S786E.Scer\UAS.P\T driven by Scer\GAL4tub.PU rescues larval development until late wandering third instar larval stage (though larvae take 7-12 extra days to reach this stage and then die shortly after) in Dis31/Dis31 mutants.

      Expression of Dis3G5039 driven by Scer\GAL4Act.PU rescues over proliferation of follicle cell phenotypes in Dis3G5039/Dis31 egg chambers.

      (Snee et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Dis31
      (Villa et al., 2024)
      dis31
      (Snee et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)