Gitex10/Gitf03586 or Gitex10/Df(2R)BSC596 flies have significant defects in climbing ability compared to controls.
Postsynaptic glutamate receptor fields are enlarged in neuromuscular junctions (NMJ) of Gitex10/Gitf03586 third instar larvae. Gitex10/Gitf03586 third instar larval NMJs (type 1b) are similar to controls in terms of synaptic vesicle number, docked vesicle number, active zone size or T-bar morphology; however, stimulated mutants display an accumulation of endosomal structures and vacuoles (>80nm vesicles or cisternae).
Gitex10/Gitf03586 third instar larval NMJs show significant reductions in evoked excitatory junctional currents (eEJCs) compared to controls (no difference in rise times or decay constants).
Gitex10/Gitf03586 or Gitex10/Df(2R)BSC596 larval NMJs show defects in synaptic vesicle recycling, with Gitex10/Gitf03586 mutants having specific defects in the slow phase of endocytosis and synaptic vesicle reacidification. Under conditions of elevated extracellular calcium, evoked neurotransmission of Gitex10/Gitf03586 larvae is similar to controls but FM1-43 uptake is significantly reduced (impaired endocytosis or synaptic vesicle recycling).