FB2025_01 , released February 20, 2025
Allele: Dmel\danyex11
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General Information
Symbol
Dmel\danyex11
Species
D. melanogaster
Name
FlyBase ID
FBal0320406
Feature type
allele
Associated gene
Dmel\dany
Associated Insertion(s)
P{?GSV7}danyex11
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
Delta2-3 transposase
(Trost et al., 2016)
Progenitor genotype
P{GSV7}danyGS22077
(Trost et al., 2016)
Associated Insertion(s)
P{?GSV7}danyex11
Cytology
Description

Deletion that removes the entire dany coding sequence. 16bp of residual P{GSV7} sequence remains.

(Trost et al., 2016)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
2R:21,984,651..21,986,825 [-]
Comment:

A 2175 bp deletion resulting from the imprecise excision of P{GSV7} removes the dany CDS.

(Lehner, 2017.2.1)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      danyex11/Df(2R)BSC802 adults are viable but males are completely sterile with abnormal testes: they are smaller in size than wild-type, full of spermatocytes and do not contain any sperm tail bundles. danyex11/Df(2R)BSC802 males also display meiotic division defects. Although meiotic cells are present they lack normal centrosomes with regular number of centrioles at the opposite poles, the meiotic spindle is often kinked or multipolar and they fail to progress to anaphase.

      The nuclear lamina of danyex11/Df(2R)BSC802 stages S3 and S5 (but not S2) spermatocytes is ballon-like lacking the deformations correlating with chromatin distribution seen in wild-type.

      (Trost et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Df(2R)BSC802/danyex11 is rescued by dany+t4.45

      (Trost et al., 2016)

      Df(2R)BSC802/danyex11 is rescued by danyN.EGFP

      (Trost et al., 2016)

      Df(2R)BSC802/danyex11 is rescued by danyC.EGFP

      (Trost et al., 2016)
      Partially rescued by

      Df(2R)BSC802/danyex11 is partially rescued by danyAAA.C.EGFP

      (Trost et al., 2016)
      Not rescued by

      Df(2R)BSC802/danyex11 is not rescued by danyUAS.N.EGFP/Scer\GAL4VP16.bam

      (Trost et al., 2016)
      Comments

      The male sterility of flies is completely rescued by combination with either of the following: dany+t4.45, danyN.T:Avic\GFP-EGFP or danyC.T:Avic\GFP-EGFP but not by combination with danyAAA.C.T:Avic\GFP-EGFP or expression of danyScer\UAS.N.T:Avic\GFP-EGFP under the control of Scer\GAL4bam.T:Hsim\VP16 in the mutant background.

      dany+t4.45 also rescues the morphological defects in spermatocyte nuclear lamina of danyex11/Df(2R)BSC802 adult males, danyC.T:Avic\GFP-EGFP rescues their testes defects: spermatogenesis is able to progress beyond spermatocyte stages and spermatids with elongated sperm tails are observed, which are significantly ameliorated also by combination with danyAAA.C.T:Avic\GFP-EGFP (spermatogenesis is able to progress beyond spermatocyte stages and spermatids with elongated sperm tails are observed but seminal vesicles are always devoid of motile sperm).

      (Trost et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      danyex11
      (Lehner, 2017.2.1, Trost et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)