FlyBase Allele Report: Bant\lef[UAS.cGa]

General Information

Symbol

Bant\lef^UAS.cGa

Species

B. anthracis

Name

FlyBase ID

FBal0321162

Feature type

allele

Associated gene

Bant\lef

Associated Insertion(s)

Carried in Construct

P{UAS-LF.G}

Also Known As

UAS-LF

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Guichard et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-LF.G}

Cytology

Description

UAS regulatory sequences drive expression of Bant\lef coding sequences where the bacterial signal sequences, comprising the first 33 amino acid residues have been removed and are replaced with a methionine preceded by an optimal Drosophila Kozak's sequence (GCCAGC).

(Guichard et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Bant\lef

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^Act.PU

(Guichard et al., 2006)

lethal, with Scer\GAL4^tsh-md621

(Guichard et al., 2006)

lethal, with Scer\GAL4^twi.PG

(Guichard et al., 2006)

partially lethal - majority die, with Scer\GAL4^{VP16.mat.αTub67C}

(Guichard et al., 2006)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Zzzz\lef^Scer\UAS.cGa in the wing disc primordium under the control of Scer\GAL4^bs-1348 produces no obvious phenotype.

Expression of Zzzz\lef^Scer\UAS.cGa in the wing disc primordium under the control of Scer\GAL4^Bx-MS1096 produces no obvious phenotype.

(Guichard et al., 2010)

Expression of Zzzz\lef^Scer\UAS.cGa under the control of Scer\GAL4^tsh-md621, Scer\GAL4^twi.PG or Scer\GAL4^Act.PU results in almost complete lethality.

Expression of Zzzz\lef^Scer\UAS.cGa under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} results in near complete lethality. Embryos exhibit a range of developmental defects. The least severely affected embryos have a U-shaped phenotype, which is typical of mutations interfering with dorsal closure. More severely affected embryos are greatly reduced in size, have dorsal holes in the cuticle, and lack all head skeletal structures. In addition, these embryos exhibit severe defects in the F-actin network in leading-edge cells, which forms a regular line in wild-type embryos.

Expression of Zzzz\lef^Scer\UAS.cGa under the control of Scer\GAL4^pnr-MD237 results in a dorsal cleft phenotype.

Strong expression of Zzzz\lef^Scer\UAS.cGa in the wing disc under the control of Scer\GAL4^Bx-MS1096 causes small scooped wings, which are elongated along the proximal-distal axis and distal vein truncations. Males with three copies of the Zzzz\lef^Scer\UAS.cGa transgene, driven by Scer\GAL4^Bx-MS1096 at 29[o]C exhibit the small wing vein phenotype with almost 100% penetrance, approximately 50% of these individuals also exhibit vein truncations.

(Guichard et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Bant\lef^UAS.cGa has wing phenotype, enhanceable by Dsor1^r1/Scer\GAL4^Bx-MS1096

(Guichard et al., 2006)

Bant\lef^UAS.cGa has wing vein phenotype, enhanceable by Dsor1^r1/Scer\GAL4^Bx-MS1096

(Guichard et al., 2006)

Suppressor of

Statement

Reference

Scer\GAL4^CY2/Bant\lef^UAS.cGa is a suppressor of wing vein L5 phenotype of hep^CA.UAS

(Guichard et al., 2006)

Scer\GAL4^CY2/Bant\lef^UAS.cGa is a suppressor of wing vein | ectopic phenotype of Raf^Act.UAS

(Guichard et al., 2006)

NOT Suppressor of

Statement

Reference

Bant\lef^UAS.cGa, Scer\GAL4^Bx-MS1096 is a non-suppressor of wing vein | ectopic phenotype of Scer\GAL4^Bx-MS1096, rl^Sem.cUa.UAS

(Guichard et al., 2006)

Other

Statement

Reference

Bant\EF^UAS.cGa, Bant\lef^UAS.cGa, Scer\GAL4^bs-1348 has wing margin phenotype

(Guichard et al., 2010)

Bant\EF^UAS.cGa, Bant\lef^UAS.cGa, Scer\GAL4^Bx-MS1096 has wing margin phenotype

(Guichard et al., 2010)

Bant\lef^UAS.cGa, Rab11^{S25N.UASp.YFP}, Scer\GAL4^Bx-MS1096 has wing margin phenotype

(Guichard et al., 2010)

Bant\lef^UAS.cGa, Scer\GAL4^Bx-MS1096, kni^ri-1 has wing vein phenotype

(Guichard et al., 2006)

Bant\lef^UAS.cGa, Scer\GAL4^Bx-MS1096, rho^ve-1 has wing vein phenotype

(Guichard et al., 2006)

Bant\lef^UAS.cGa, Scer\GAL4^Bx-MS1096, vn^unspecified has wing vein phenotype

(Guichard et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Zzzz\EF^Scer\UAS.cGa and Zzzz\lef^Scer\UAS.cGa in the wing disc primordium under the control of Scer\GAL4^bs-1348 produces a strong and penetrant wing margin notching phenotype.

Co-expression of Zzzz\EF^Scer\UAS.cGa and Zzzz\lef^Scer\UAS.cGa in the wing disc primordium under the control of Scer\GAL4^Bx-MS1096 produces a strong and penetrant wing margin notching phenotype.

Co-expression of Rab11^{S25N.Scer\UAS.P\T.T:Avic\GFP-YFP} with Zzzz\lef^Scer\UAS.cGa in the wing disc primordium produces a strong and penetrant wing margin notching phenotype (when both are under the control of Scer\GAL4^Bx-MS1096).

(Guichard et al., 2010)

Moderate expression of Bant\lef^UAS.cGa under the control of Scer\GAL4^Bx-MS1096 in rho^ve-1 heterozygotes results in wing vein loss, while moderate Bant\lef^UAS.cGa expression (with Scer\GAL4^Bx-MS1096) alone doesn't have this phenotype.

Moderate expression of Bant\lef^UAS.cGa under the control of Scer\GAL4^Bx-MS1096 in vn^unspecified heterozygotes results in wing vein loss, while moderate Bant\lef^UAS.cGa expression (with Scer\GAL4^Bx-MS1096) alone doesn't have this phenotype.

Moderate expression of Bant\lef^UAS.cGa under the control of Scer\GAL4^Bx-MS1096 in kni^ri-1 heterozygotes results in wing vein loss, while moderate Bant\lef^UAS.cGa expression (with Scer\GAL4^Bx-MS1096) alone doesn't have this phenotype.

Expression of Bant\lef^UAS.cGa does not suppress the ectopic vein phenotype of rl^Sem.cUa.UAS under the control of Scer\GAL4^Bx-MS1096.

Expression of Bant\lef^UAS.cGa suppresses the strong ectopic vein phenotype of Raf^Act.UAS under the control of Scer\GAL4^CY2.

Moderate expression of Bant\lef^UAS.cGa under the control of Scer\GAL4^Bx-MS1096 in Dsor1^r1 results in an aggravated Bant\lef^UAS.cGa (Scer\GAL4^Bx-MS1096) phenotype consisting of more pronounced scooped wings and distal vein truncations.

(Guichard et al., 2006)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Bant\lef^Scer\UAS.cGa

Bant\lef^UAS.cGa

Zzzz\lef^Scer\UAS.cGa

Name Synonyms

Secondary FlyBase IDs

FBal0320548
FBal0247339

References (3)

Report Sections

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