FlyBase Allele Report: Asam\aopP[UAS.Tag:MYC]

General Information

Symbol

Asam\aopP^UAS.Tag:MYC

Species

A. salmonicida

Name

FlyBase ID

FBal0321238

Feature type

allele

Associated gene

Asam\aopP

Associated Insertion(s)

Carried in Construct

P{UAS-aopP.myc}

Also Known As

UAS-aopP

Transgenic product class

wild_type

(Jones et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Jones et al., 2012)

Mutagen

in vitro construct

(Jones et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-aopP.myc}

Cytology

Description

UASt regulatory sequences drive expression of a Tag:MYC-tagged Asam\aopP.

(Jones et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Asam\aopP

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response, with Scer\GAL4^Hml.PG

(Jones et al., 2012)

increased cell death | third instar larval stage, with Scer\GAL4^c564

(Jones et al., 2012)

increased cell death | third instar larval stage, with Scer\GAL4^hs.PU

(Jones et al., 2012)

lethal | heat sensitive, with Scer\GAL4^hs.PU

(Jones et al., 2012)

visible, with Scer\GAL4^bbg-C96

(Jones et al., 2012)

visible, with Scer\GAL4^GMR.PF

(Jones et al., 2012, Jones et al., 2008)

Phenotype Manifest In

embryonic/larval plasmatocyte, with Scer\GAL4^Hml.PG

(Jones et al., 2012)

eye, with Scer\GAL4^GMR.PF

(Jones et al., 2012, Jones et al., 2008)

fat body, with Scer\GAL4^c564

(Jones et al., 2012)

fat body, with Scer\GAL4^hs.PU

(Jones et al., 2012)

wing margin, with Scer\GAL4^bbg-C96

(Jones et al., 2012)

Detailed Description

Statement

Reference

Expression of Zzzz\aopP^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^hs.PU results in complete loss of viability up to 96 hours post heat-shock.

Monitoring of survival reveals no significant increase in mortality rate between uninfected Zzzz\aopP^{Scer\UAS.T:Hsap\MYC}-expressing flies and isogenic controls injured with a sterile needle. However, heat-shock induced expression of Zzzz\aopP^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^hs.PU significantly dampens the ability of flies to respond to both Gram-negative and Gram-positive bacteria compared with controls. Bacterially infected Zzzz\aopP^{Scer\UAS.T:Hsap\MYC}-expressing flies exhibit significantly increased mortality rates compared with isogenic Zzzz\aopP^{Scer\UAS.T:Hsap\MYC}-expressing flies injured with a sterile needle. No significant differences are detected between the survival rates of infected Zzzz\aopP^{Scer\UAS.T:Hsap\MYC}-expressing flies compared with infected controls expressing only the Scer\GAL4^hs.PU driver.

Expression of Zzzz\aopP^{Scer\UAS.T:Hsap\MYC} in the eye under using Scer\GAL4^GMR.PF results in an altered eye phenotype compared with controls.

Adult flies expressing Zzzz\aopP^{Scer\UAS.T:Hsap\MYC} in the wing margin using Scer\GAL4^bbg-C96 exhibit dose dependant wing margin notching.

Fat bodies expressing Zzzz\aopP^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^c564 exhibit increased apoptosis compared with control larvae.

Fat bodies expressing Zzzz\aopP^{Scer\UAS.T:Hsap\MYC} 72 hours after heat-shock under the control of Scer\GAL4^hs.PU exhibit increased apoptosis compared with control larvae.

Expression of Zzzz\aopP^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Hml.PG results in about a 60 % reduction in the number of plasmatocytes compared with controls.

Flies expressing Zzzz\aopP^{Scer\UAS.T:Hsap\MYC} in plasmatocytes under the control of Scer\GAL4^Hml.PG show significantly reduced survival rate following parental infection with Micrococcus luteus only between 5 and 8 days post infection, compared with control flies. Zzzz\aopP^{Scer\UAS.T:Hsap\MYC}-expressing flies infected with Erwinia carotovora also exhibit noticeably reduced survival, compared with controls, between 5 and 8 days post infection, although these differences are not statistically significant. No significant differences are detected between Zzzz\aopP^{Scer\UAS.T:Hsap\MYC}-expressing flies and controls when stabbed with a sterile needle.

(Jones et al., 2012)

Flies expressing Zzzz\aopP^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF exhibit a rough eye phenotype.

(Jones et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Asam\aopP^UAS.Tag:MYC, Scer\GAL4^GMR.PF has visible phenotype, non-enhanceable by Ras85D^V12.cUa.UAS, Scer\GAL4^GMR.PF

(Jones et al., 2012)

Asam\aopP^UAS.Tag:MYC, Scer\GAL4^GMR.PF has visible phenotype, non-enhanceable by rl^Sem.cUa.UAS, Scer\GAL4^GMR.PF

(Jones et al., 2012)

Asam\aopP^UAS.Tag:MYC, Scer\GAL4^GMR.PF has visible phenotype, non-enhanceable by pnt^P2.UAS, Scer\GAL4^GMR.PF

(Jones et al., 2012)

Suppressed by

Statement

Reference

Asam\aopP^UAS.Tag:MYC, Scer\GAL4^GMR.PF has visible phenotype, suppressible by Diap1^UAS.cUa, Scer\GAL4^GMR.PF

(Jones et al., 2012, Jones et al., 2008)

Asam\aopP^UAS.Tag:MYC, Scer\GAL4^bbg-C96 has visible phenotype, suppressible by BacA\p35^UAS.cUa, Scer\GAL4^bbg-C96

(Jones et al., 2012)

Asam\aopP^UAS.Tag:MYC, Scer\GAL4^GMR.PF has visible phenotype, suppressible by BacA\p35^UAS.cUa, Scer\GAL4^GMR.PF

(Jones et al., 2008)

NOT suppressed by

Statement

Reference

Asam\aopP^UAS.Tag:MYC, Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by Ras85D^V12.cUa.UAS, Scer\GAL4^GMR.PF

(Jones et al., 2012)

Asam\aopP^UAS.Tag:MYC, Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by rl^Sem.cUa.UAS, Scer\GAL4^GMR.PF

(Jones et al., 2012)

Asam\aopP^UAS.Tag:MYC, Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by pnt^P2.UAS, Scer\GAL4^GMR.PF

(Jones et al., 2012)

Suppressor of

Statement

Reference

Asam\aopP^UAS.Tag:MYC, Scer\GAL4^GMR.PF is a suppressor | partially of visible phenotype of Scer\GAL4^GMR.PF, egr^UAS.cUa

(Jones et al., 2012)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Asam\aopP^UAS.Tag:MYC, Scer\GAL4^GMR.PF has eye phenotype, non-enhanceable by Ras85D^V12.cUa.UAS, Scer\GAL4^GMR.PF

(Jones et al., 2012)

Asam\aopP^UAS.Tag:MYC, Scer\GAL4^GMR.PF has eye phenotype, non-enhanceable by rl^Sem.cUa.UAS, Scer\GAL4^GMR.PF

(Jones et al., 2012)

Asam\aopP^UAS.Tag:MYC, Scer\GAL4^GMR.PF has eye phenotype, non-enhanceable by pnt^P2.UAS, Scer\GAL4^GMR.PF

(Jones et al., 2012)

Suppressed by

Statement

Reference

Asam\aopP^UAS.Tag:MYC, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Diap1^UAS.cUa, Scer\GAL4^GMR.PF

(Jones et al., 2012, Jones et al., 2008)

Asam\aopP^UAS.Tag:MYC, Scer\GAL4^bbg-C96 has wing margin phenotype, suppressible by BacA\p35^UAS.cUa, Scer\GAL4^bbg-C96

(Jones et al., 2012)

Asam\aopP^UAS.Tag:MYC, Scer\GAL4^GMR.PF has eye phenotype, suppressible by BacA\p35^UAS.cUa, Scer\GAL4^GMR.PF

(Jones et al., 2008)

NOT suppressed by

Statement

Reference

Asam\aopP^UAS.Tag:MYC, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by Ras85D^V12.cUa.UAS, Scer\GAL4^GMR.PF

(Jones et al., 2012)

Asam\aopP^UAS.Tag:MYC, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by rl^Sem.cUa.UAS, Scer\GAL4^GMR.PF

(Jones et al., 2012)

Asam\aopP^UAS.Tag:MYC, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by pnt^P2.UAS, Scer\GAL4^GMR.PF

(Jones et al., 2012)

Suppressor of

Statement

Reference

Asam\aopP^UAS.Tag:MYC, Scer\GAL4^GMR.PF is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PF, egr^UAS.cUa

(Jones et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of th^Scer\UAS.cUa using Scer\GAL4^GMR.PF markedly suppresses the eye phenotype caused by the expression of Zzzz\aopP^{Scer\UAS.T:Hsap\MYC}.

Co-expression of BacA\p35^Scer\UAS.cUa using Scer\GAL4^bbg-C96 completely suppresses the wing notching phenotype caused by the expression of Zzzz\aopP^{Scer\UAS.T:Hsap\MYC}.

Co-expression of Ras85D^{V12.cUa.Scer\UAS} with Zzzz\aopP^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF does not alter the eye morphology of Zzzz\aopP^{Scer\UAS.T:Hsap\MYC}-expressing eyes.

Co-expression of rl^{sem.cUa.Scer\UAS} with Zzzz\aopP^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF does not alter the eye morphology of Zzzz\aopP^{Scer\UAS.T:Hsap\MYC}-expressing eyes.

Co-expression of pnt^P2.Scer\UAS with Zzzz\aopP^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF does not alter the eye morphology of Zzzz\aopP^{Scer\UAS.T:Hsap\MYC}-expressing eyes.

Co-expression of Zzzz\aopP^{Scer\UAS.T:Hsap\MYC} with egr^Scer\UAS.cUa under the control of Scer\GAL4^GMR.PF partially rescues the eye phenotype resulting from the overexpression of egr^Scer\UAS.cUa. While the size of the Zzzz\aopP^{Scer\UAS.T:Hsap\MYC}-rescued eye is marginally larger than the eyes expressing egr^Scer\UAS.cUa alone, black necrotic tissue is clearly visible within the eye tissue.

(Jones et al., 2012)

Expression of BacA\p35^Scer\UAS.cUa suppresses the rough eye phenotype seen when Zzzz\aopP^{Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^GMR.PF.

Expression of th^Scer\UAS.cUa suppresses the rough eye phenotype seen when Zzzz\aopP^{Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^GMR.PF.

(Jones et al., 2008)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Asam\aopP^{Scer\UAS.T:Hsap\MYC}

Asam\aopP^UAS.Tag:MYC

Zzzz\aopP^{Scer\UAS.T:Hsap\MYC}

Name Synonyms

Secondary FlyBase IDs

FBal0320624
FBal0276791

References (3)

Report Sections

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