FlyBase Allele Report: Besp\bPAC[UAS.cSa]

General Information

Symbol

Besp\bPAC^UAS.cSa

Species

B. sp.

Name

FlyBase ID

FBal0321272

Feature type

allele

Associated gene

Besp\bPAC

Associated Insertion(s)

Carried in Construct

P{UAS-bPAC.S}

Also Known As

UAS-bPAC

Transgenic product class

cDNA

(Stierl et al., 2011)

wild_type

(Stierl et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Stierl et al., 2011)

wild_type

(Stierl et al., 2011)

Mutagen

in vitro construct

(Stierl et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-bPAC.S}

Cytology

Description

UASt regulatory sequences drive expression of a Besp\bPAC cDNA.

(Stierl et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Besp\bPAC

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior, with Scer\GAL4^elav.PU

(Stierl et al., 2011)

abnormal neurophysiology | larval stage | conditional, with Scer\GAL4^iav.PP

(Scholz et al., 2017)

abnormal sensory perception | larval stage | conditional, with Scer\GAL4^iav.PP

(Scholz et al., 2017)

Phenotype Manifest In

larval abdominal lch5 neuron | conditional, with Scer\GAL4^iav.PP

(Scholz et al., 2017)

Malpighian tubule, with Scer\GAL4^c42

(Efetova et al., 2013)

Malpighian tubule, with Scer\GAL4^c42, Scer\GAL4^tsh-c724

(Efetova et al., 2013)

Malpighian tubule | conditional, with Scer\GAL4^c42

(Efetova et al., 2013)

Malpighian tubule | conditional, with Scer\GAL4^tsh-c724

(Efetova et al., 2013)

Detailed Description

Statement

Reference

Expression of Zzzz\bPAC^Scer\UAS.cSa under the control of Scer\GAL4^c42 in the absence of blue light stimulation results in an increase in fluid secretion from the Malpighian tubules compared to controls. The addition of the Scer\GAL4^tsh-c724 driver further increases the secretion rate (expression under the control of the Scer\GAL4^tsh-c724 driver alone does not increase the secretion rate above that of controls).

Expression of Zzzz\bPAC^Scer\UAS.cSa under the control of Scer\GAL4^tsh-c724 with light activation of low/intermediate intensities results in stimulation of fluid secretion from the Malpighian tubules, whereas high intensity light stimulation does not affect fluid secretion.

Expression of Zzzz\bPAC^Scer\UAS.cSa under the control of Scer\GAL4^c42 stimulates fluid secretion from the Malpighian tubules at a light intensity of 0.2 mW/cm[2], but higher intensities of light activation result in inhibition of fluid secretion.

(Efetova et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Besp\bPAC^UAS.cSa, Scer\GAL4^c42 has Malpighian tubule | conditional phenotype, enhanceable by Epac^UAS.cEa, Scer\GAL4^c42

(Efetova et al., 2013)

Besp\bPAC^UAS.cSa, Scer\GAL4^tsh-c724 has Malpighian tubule | conditional phenotype, enhanceable by Epac^UAS.cEa, Scer\GAL4^tsh-c724

(Efetova et al., 2013)

Suppressed by

Statement

Reference

Besp\bPAC^UAS.cSa, Scer\GAL4^tsh-c724 has Malpighian tubule | conditional phenotype, suppressible by Pka-C1^VDRC.cUa, Scer\GAL4^tsh-c724

(Efetova et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Pka-C1^VDRC.cUa completely abolishes the light-activated stimulation of fluid secretion from the Malpighian tubules which is caused by expression of Zzzz\bPAC^Scer\UAS.cSa under the control of Scer\GAL4^tsh-c724.

Animals expressing Zzzz\bPAC^Scer\UAS.cSa under the control of Scer\GAL4^c42 in a Pka-C1^B10/+ background show significant stimulation of fluid secretion from the Malpighian tubules in response to low light activation.

Animals expressing Zzzz\bPAC^Scer\UAS.cSa under the control of Scer\GAL4^c42 and also co-expressing Pka-C1^VDRC.cUa show significant stimulation of fluid secretion from the Malpighian tubules in response to low light activation.

Co-expression of Epac^Scer\UAS.cEa elevates light activated stimulation of fluid secretion from the Malpighian tubules caused by expression of Zzzz\bPAC^Scer\UAS.cSa under the control of Scer\GAL4^c42, but has no effect on basal secretion rates.

Co-expression of Epac^Scer\UAS.cEa elevates both basal and stimulated fluid secretion from the Malpighian tubules caused by expression of Zzzz\bPAC^Scer\UAS.cSa under the control of Scer\GAL4^tsh-c724.

(Efetova et al., 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

78787

P{UAS-bPAC.S}1, w^*

78788

w^*; P{UAS-bPAC.S}2/CyO