FlyBase Allele Report: Spyo\Cas9[UAS.Tag:NLS(Unk)]

General Information

Symbol

Spyo\Cas9^{UAS.Tag:NLS(Unk)}

Species

S. pyogenes

Name

FlyBase ID

FBal0321288

Feature type

allele

Associated gene

Spyo\Cas9

Associated Insertion(s)

Carried in Construct

P{UAS-Cas9.C}

Also Known As

UAS-Cas9, UAS-Cas9.C

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Port and Bullock, 2014.1.10)

in vitro construct - coding region fusion

(Port and Bullock, 2014.1.10)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{UAS-Cas9.C}

Cytology

Nature of the lesion

Statement

Reference

A Drosophila codon-optimized Cas9 endonuclease with N- and C-terminal nuclear localization signal sequences is expressed under the control of UAS regulatory sequences (ORF cloned into the KpnI and XbaI restriction sites of P{10XUAS-IVS-GFP-p10}, FBtp0073105).

(Port and Bullock, 2014.1.10)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Cas9

RNA-guided nuclease

Tagged with

Tag:NLS(Unk)

nuclear protein localization tag

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

lethal, with EcR^gRNA.pCFD3-1

(Meltzer et al., 2019)

lethal, with EcR^gRNA.pCFD3-1, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

lethal, with EcR^gRNA.pCFD3-2

(Meltzer et al., 2019)

lethal, with EcR^gRNA.pCFD3-2, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

lethal, with EcR^gRNA.pCFD3-3

(Meltzer et al., 2019)

lethal, with EcR^gRNA.pCFD3-3, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

lethal, with Rpn8^gRNA.pCFD3-1

(Meltzer et al., 2019)

lethal, with Rpn8^gRNA.pCFD3-1, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

lethal, with Rpn8^gRNA.pCFD3-2

(Meltzer et al., 2019)

lethal, with Rpn8^gRNA.pCFD3-2, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

lethal, with Rpn8^gRNA.pCFD3-3

(Meltzer et al., 2019)

lethal, with Rpn8^gRNA.pCFD3-3, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

locomotor behavior defective, with Pzl^U6.sgRNA, Scer\GAL4^iav.PP

(Hu et al., 2019)

neuroanatomy defective | adult stage, with Eip75B^gRNA.pCFD3-1, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

neuroanatomy defective | adult stage, with Eip75B^gRNA.pCFD3-2, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

neuroanatomy defective | adult stage, with Eip75B^gRNA.pCFD3-3, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

neuroanatomy defective | adult stage, with Rpn6^gRNA.pCFD3-1, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

neuroanatomy defective | adult stage, with Rpn6^gRNA.pCFD3-2, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

neuroanatomy defective | adult stage, with Rpn6^gRNA.pCFD3-3, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

neuroanatomy defective | adult stage, with Scer\GAL4^GMR71G10, bsk^gRNA.pCFD3-1

(Meltzer et al., 2019)

neuroanatomy defective | adult stage, with Scer\GAL4^GMR71G10, bsk^gRNA.pCFD3-2

(Meltzer et al., 2019)

neuroanatomy defective | adult stage, with Scer\GAL4^GMR71G10, bsk^gRNA.pCFD3-3

(Meltzer et al., 2019)

neuroanatomy defective | adult stage, with Scer\GAL4^GMR71G10, plum^gRNA.pCFD3-1

(Meltzer et al., 2019)

neuroanatomy defective | adult stage, with Scer\GAL4^GMR71G10, plum^gRNA.pCFD3-2

(Meltzer et al., 2019)

neuroanatomy defective | adult stage, with Scer\GAL4^GMR71G10, plum^gRNA.pCFD3-3

(Meltzer et al., 2019)

neuroanatomy defective | adult stage, with Scer\GAL4^GMR71G10, plum^{UAS.gRNAx2.pCFD6}

(Meltzer et al., 2019)

neuroanatomy defective | adult stage, with Scer\GAL4^GMR71G10, plum^WKO.1-F11

(Meltzer et al., 2019)

neuroanatomy defective | adult stage, with Scer\GAL4^GMR71G10, plum^WKO.5-D2

(Meltzer et al., 2019)

neuroanatomy defective | adult stage, with Scer\GAL4^GMR71G10, Uba1^gRNA.pCFD3-1

(Meltzer et al., 2019)

neuroanatomy defective | adult stage, with Scer\GAL4^GMR71G10, Uba1^gRNA.pCFD3-2

(Meltzer et al., 2019)

neuroanatomy defective | adult stage, with Scer\GAL4^GMR71G10, Uba1^gRNA.pCFD3-3

(Meltzer et al., 2019)

neuroanatomy defective | adult stage, with Scer\GAL4^GMR71G10, usp^gRNA.pCFD3-1

(Meltzer et al., 2019)

neuroanatomy defective | adult stage, with Scer\GAL4^GMR71G10, usp^gRNA.pCFD3-2

(Meltzer et al., 2019)

neuroanatomy defective | adult stage, with Scer\GAL4^GMR71G10, usp^gRNA.pCFD3-3

(Meltzer et al., 2019)

neuroanatomy defective | adult stage, with Scer\GAL4^GMR71G10, Uvrag^gRNA.pCFD3-1

(Meltzer et al., 2019)

neuroanatomy defective | adult stage, with Scer\GAL4^GMR71G10, Uvrag^gRNA.pCFD3-2

(Meltzer et al., 2019)

neuroanatomy defective | adult stage, with Scer\GAL4^GMR71G10, Uvrag^gRNA.pCFD3-3

(Meltzer et al., 2019)

neuroanatomy defective | pupal stage, with CG9003^WKO.1-H5, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

neuroanatomy defective | pupal stage, with CG34353^WKO.3-A2, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

neuroanatomy defective | pupal stage, with Dop1R2^WKO.1-C1, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

neuroanatomy defective | pupal stage, with Mmp2^WKO.3-C7, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

neuroanatomy defective | pupal stage, with Pli^WKO.1-C10, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

neuroanatomy defective | pupal stage, with Scer\GAL4^GMR71G10, danr^WKO.3-B8

(Meltzer et al., 2019)

neuroanatomy defective | pupal stage, with Scer\GAL4^GMR71G10, hppy^WKO.1-F10

(Meltzer et al., 2019)

neuroanatomy defective | pupal stage, with Scer\GAL4^GMR71G10, mamo^gRNA.pCFD3

(Meltzer et al., 2019)

neuroanatomy defective | pupal stage, with Scer\GAL4^GMR71G10, mamo^{UAS.gRNAx2.pCFD6}

(Meltzer et al., 2019)

neuroanatomy defective | pupal stage, with Scer\GAL4^GMR71G10, mamo^WKO.5-A2

(Meltzer et al., 2019)

neuroanatomy defective | pupal stage, with Scer\GAL4^GMR71G10, mamo^WKO.5-B2

(Meltzer et al., 2019)

partially lethal | maternal effect, with Scer\GAL4^{VP16.mat.αTub67C}, Trpm^U6:3.gRNA1

(Hu and Wolfner, 2019)

some die during embryonic stage | maternal effect, with Scer\GAL4^{VP16.mat.αTub67C}, Trpm^U6:3.gRNA1

(Hu and Wolfner, 2019)

viable, with CG9003^WKO.1-H5, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

viable, with CG34353^WKO.3-A2, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

viable, with Dop1R2^WKO.1-C1, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

viable, with Mmp2^WKO.3-C7, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

viable, with Pli^WKO.1-C10, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

viable, with Scer\GAL4^GMR71G10, danr^WKO.3-B8

(Meltzer et al., 2019)

viable, with Scer\GAL4^GMR71G10, hppy^WKO.1-F10

(Meltzer et al., 2019)

visible, with Scer\GAL4^en.PU, wls^{gRNA.snRNA:U6:96Ac}

(Port et al., 2014)

Phenotype Manifest In

axon | adult stage, with Eip75B^gRNA.pCFD3-1, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

axon | adult stage, with Eip75B^gRNA.pCFD3-2, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

axon | adult stage, with Eip75B^gRNA.pCFD3-3, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

axon | adult stage, with Rpn6^gRNA.pCFD3-1, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

axon | adult stage, with Rpn6^gRNA.pCFD3-2, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

axon | adult stage, with Rpn6^gRNA.pCFD3-3, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

axon | adult stage, with Scer\GAL4^GMR71G10, bsk^gRNA.pCFD3-1

(Meltzer et al., 2019)

axon | adult stage, with Scer\GAL4^GMR71G10, bsk^gRNA.pCFD3-2

(Meltzer et al., 2019)

axon | adult stage, with Scer\GAL4^GMR71G10, bsk^gRNA.pCFD3-3

(Meltzer et al., 2019)

axon | adult stage, with Scer\GAL4^GMR71G10, plum^gRNA.pCFD3-1

(Meltzer et al., 2019)

axon | adult stage, with Scer\GAL4^GMR71G10, plum^gRNA.pCFD3-2

(Meltzer et al., 2019)

axon | adult stage, with Scer\GAL4^GMR71G10, plum^gRNA.pCFD3-3

(Meltzer et al., 2019)

axon | adult stage, with Scer\GAL4^GMR71G10, plum^{UAS.gRNAx2.pCFD6}

(Meltzer et al., 2019)

axon | adult stage, with Scer\GAL4^GMR71G10, plum^WKO.1-F11

(Meltzer et al., 2019)

axon | adult stage, with Scer\GAL4^GMR71G10, plum^WKO.5-D2

(Meltzer et al., 2019)

axon | adult stage, with Scer\GAL4^GMR71G10, Uba1^gRNA.pCFD3-1

(Meltzer et al., 2019)

axon | adult stage, with Scer\GAL4^GMR71G10, Uba1^gRNA.pCFD3-2

(Meltzer et al., 2019)

axon | adult stage, with Scer\GAL4^GMR71G10, Uba1^gRNA.pCFD3-3

(Meltzer et al., 2019)

axon | adult stage, with Scer\GAL4^GMR71G10, usp^gRNA.pCFD3-1

(Meltzer et al., 2019)

axon | adult stage, with Scer\GAL4^GMR71G10, usp^gRNA.pCFD3-2

(Meltzer et al., 2019)

axon | adult stage, with Scer\GAL4^GMR71G10, usp^gRNA.pCFD3-3

(Meltzer et al., 2019)

axon | adult stage, with Scer\GAL4^GMR71G10, Uvrag^gRNA.pCFD3-1

(Meltzer et al., 2019)

axon | adult stage, with Scer\GAL4^GMR71G10, Uvrag^gRNA.pCFD3-2

(Meltzer et al., 2019)

axon | adult stage, with Scer\GAL4^GMR71G10, Uvrag^gRNA.pCFD3-3

(Meltzer et al., 2019)

axon | pupal stage, with CG9003^WKO.1-H5, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

axon | pupal stage, with CG34353^WKO.3-A2, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

axon | pupal stage, with Dop1R2^WKO.1-C1, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

axon | pupal stage, with Mmp2^WKO.3-C7, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

axon | pupal stage, with Pli^WKO.1-C10, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

axon | pupal stage, with Scer\GAL4^GMR71G10, danr^WKO.3-B8

(Meltzer et al., 2019)

axon | pupal stage, with Scer\GAL4^GMR71G10, hppy^WKO.1-F10

(Meltzer et al., 2019)

axon | pupal stage, with Scer\GAL4^GMR71G10, mamo^gRNA.pCFD3

(Meltzer et al., 2019)

axon | pupal stage, with Scer\GAL4^GMR71G10, mamo^{UAS.gRNAx2.pCFD6}

(Meltzer et al., 2019)

axon | pupal stage, with Scer\GAL4^GMR71G10, mamo^WKO.5-A2

(Meltzer et al., 2019)

axon | pupal stage, with Scer\GAL4^GMR71G10, mamo^WKO.5-B2

(Meltzer et al., 2019)

egg, with Scer\GAL4^{VP16.mat.αTub67C}, Trpm^U6:3.gRNA1

(Hu and Wolfner, 2019)

gamma Kenyon cell | adult stage, with Eip75B^gRNA.pCFD3-1, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

gamma Kenyon cell | adult stage, with Eip75B^gRNA.pCFD3-2, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

gamma Kenyon cell | adult stage, with Eip75B^gRNA.pCFD3-3, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

gamma Kenyon cell | adult stage, with Rpn6^gRNA.pCFD3-1, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

gamma Kenyon cell | adult stage, with Rpn6^gRNA.pCFD3-2, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

gamma Kenyon cell | adult stage, with Rpn6^gRNA.pCFD3-3, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

gamma Kenyon cell | adult stage, with Scer\GAL4^GMR71G10, bsk^gRNA.pCFD3-1

(Meltzer et al., 2019)

gamma Kenyon cell | adult stage, with Scer\GAL4^GMR71G10, bsk^gRNA.pCFD3-2

(Meltzer et al., 2019)

gamma Kenyon cell | adult stage, with Scer\GAL4^GMR71G10, bsk^gRNA.pCFD3-3

(Meltzer et al., 2019)

gamma Kenyon cell | adult stage, with Scer\GAL4^GMR71G10, plum^gRNA.pCFD3-1

(Meltzer et al., 2019)

gamma Kenyon cell | adult stage, with Scer\GAL4^GMR71G10, plum^gRNA.pCFD3-2

(Meltzer et al., 2019)

gamma Kenyon cell | adult stage, with Scer\GAL4^GMR71G10, plum^gRNA.pCFD3-3

(Meltzer et al., 2019)

gamma Kenyon cell | adult stage, with Scer\GAL4^GMR71G10, plum^{UAS.gRNAx2.pCFD6}

(Meltzer et al., 2019)

gamma Kenyon cell | adult stage, with Scer\GAL4^GMR71G10, plum^WKO.1-F11

(Meltzer et al., 2019)

gamma Kenyon cell | adult stage, with Scer\GAL4^GMR71G10, plum^WKO.5-D2

(Meltzer et al., 2019)

gamma Kenyon cell | adult stage, with Scer\GAL4^GMR71G10, Uba1^gRNA.pCFD3-1

(Meltzer et al., 2019)

gamma Kenyon cell | adult stage, with Scer\GAL4^GMR71G10, Uba1^gRNA.pCFD3-2

(Meltzer et al., 2019)

gamma Kenyon cell | adult stage, with Scer\GAL4^GMR71G10, Uba1^gRNA.pCFD3-3

(Meltzer et al., 2019)

gamma Kenyon cell | adult stage, with Scer\GAL4^GMR71G10, usp^gRNA.pCFD3-1

(Meltzer et al., 2019)

gamma Kenyon cell | adult stage, with Scer\GAL4^GMR71G10, usp^gRNA.pCFD3-2

(Meltzer et al., 2019)

gamma Kenyon cell | adult stage, with Scer\GAL4^GMR71G10, usp^gRNA.pCFD3-3

(Meltzer et al., 2019)

gamma Kenyon cell | adult stage, with Scer\GAL4^GMR71G10, Uvrag^gRNA.pCFD3-1

(Meltzer et al., 2019)

gamma Kenyon cell | adult stage, with Scer\GAL4^GMR71G10, Uvrag^gRNA.pCFD3-2

(Meltzer et al., 2019)

gamma Kenyon cell | adult stage, with Scer\GAL4^GMR71G10, Uvrag^gRNA.pCFD3-3

(Meltzer et al., 2019)

gamma Kenyon cell | pupal stage, with CG9003^WKO.1-H5, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

gamma Kenyon cell | pupal stage, with CG34353^WKO.3-A2, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

gamma Kenyon cell | pupal stage, with Dop1R2^WKO.1-C1, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

gamma Kenyon cell | pupal stage, with Mmp2^WKO.3-C7, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

gamma Kenyon cell | pupal stage, with Pli^WKO.1-C10, Scer\GAL4^GMR71G10

(Meltzer et al., 2019)

gamma Kenyon cell | pupal stage, with Scer\GAL4^GMR71G10, danr^WKO.3-B8

(Meltzer et al., 2019)

gamma Kenyon cell | pupal stage, with Scer\GAL4^GMR71G10, hppy^WKO.1-F10

(Meltzer et al., 2019)

gamma Kenyon cell | pupal stage, with Scer\GAL4^GMR71G10, mamo^gRNA.pCFD3

(Meltzer et al., 2019)

gamma Kenyon cell | pupal stage, with Scer\GAL4^GMR71G10, mamo^{UAS.gRNAx2.pCFD6}

(Meltzer et al., 2019)

gamma Kenyon cell | pupal stage, with Scer\GAL4^GMR71G10, mamo^WKO.5-A2

(Meltzer et al., 2019)

gamma Kenyon cell | pupal stage, with Scer\GAL4^GMR71G10, mamo^WKO.5-B2

(Meltzer et al., 2019)

wing, with Scer\GAL4^en.PU, wls^{gRNA.snRNA:U6:96Ac}

(Port et al., 2014)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

54595

w¹¹¹⁸; P{UAS-Cas9.C}attP2

Notes on Origin

Discoverer

Comments

Generated by Hui-Min Chen in the laboratory of Tzumin Lee, Janelia Farm Research Campus.

(Port and Bullock, 2014.1.10)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Spyo\Cas9^{Scer\UAS.T:Uuuu\nls-Unk}

Spyo\Cas9^{UAS.Tag:NLS(Unk)}

Zzzz\Cas9^{Scer\UAS.T:Uuuu\nls-Unk}

Zzzz\Cas9^{Scer\UAS.T:Uuuu\nls6}

Name Synonyms

Secondary FlyBase IDs

FBal0320674
FBal0294143

References (9)

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