A Drosophila codon-optimized Cas9 endonuclease with N- and C-terminal nuclear localization signal sequences is expressed under the control of UAS regulatory sequences (ORF cloned into the KpnI and XbaI restriction sites of P{10XUAS-IVS-GFP-p10}, FBtp0073105).
Generated by Hui-Min Chen in the laboratory of Tzumin Lee, Janelia Farm Research Campus.