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General Information
Symbol
Dmel\jnjΔ35
Species
D. melanogaster
Name
FlyBase ID
FBal0321611
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:

1001 bp deletion resulting from the imprecise excision of P{EP}EP545 reported to mao to 3R:24192426..24193426 , R6 coordinates.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

1000bp deletion ( 3R:24192426..24193426 , release 6 genome) within jnj, removing the 5'UTR and the first three coding exons.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Eggs from jnjΔ35/jnjΔ35 mothers exhibit a significant reduction in hatching rate, as compared to wild type, and a subset of these embryos arrest at embryonic stage 2, but both of these phenotypes are suppressible when reared at 18[o]C instead of 25[o]C. Embryos also exhibit developmental delay and nuclear abnormalities that are not suppressible by rearing at 18[o]C, including nuclear degeneration and erroneous nuclear divisions; at stages 4-5 exhibit a "nuclear fallout" phenotype wherein faulty nuclei are actively removed into the yolk; after cleavage cycles these embryos exhibit frequent anaphase-bridge formation and gaps in the embryonic cortex; but no abnormalities in eggshell patterning are observed.

jnjΔ35/jnjΔ35 mutant females exhibit an increase in X chromosome, but not autosome, non-disjunction during meiosis, and this phenotype is suppressible by rearing flies at 18[o]C instead of 25[o]C; no difference in crossover rate is seen in these mutants, as compared to controls. Germaria in jnjΔ35/jnjΔ35 mutant females exhibit similar levels of DNA double stranded breaks compared to wild type, but these are insufficiently repaired in mutants, and this phenotype is suppressed when rearing flies at 18[o]C instead of 25[o]C. Ovaries of jnjΔ35/jnjΔ35 mutant females exhibit increased pachytene arrest and delayed karyosome dynamics, as compared to wild type.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

jnjΔ35 has meiotic cell cycle defective | female | temperature conditional phenotype, suppressible by mei-W682-0949/mei-W682-0949

Phenotype Manifest In
Suppressed by
Statement
Reference

jnjΔ35 has nucleus | maternal effect | embryonic stage phenotype, suppressible by lok[+]/lokp6

Additional Comments
Genetic Interactions
Statement
Reference

Embryos at stages 4-5 from lokp6/+, jnjΔ35/jnjΔ35 double mutant mothers exhibit reduced nuclear fallout as compared to those from jnjΔ35/jnjΔ35 single mutant mothers.

mei-W682-0949/mei-W682-0949, jnjΔ35/jnjΔ35 double mutants exhibit suppression of the pachytene arrest and delay in karyosome dynamics, as compared to jnjΔ35/jnjΔ35 single mutants.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by

jnjΔ35 is partially rescued by jnjTag:HA

jnjΔ35 is partially rescued by jnjTag:HA

Comments

Expression of jnjT:Ivir\HA1 partially rescues the lethality, developmental arrest, nuclear fallout, and anaphase-bridge formation phenotypes exhibited by embryos from jnjΔ35/jnjΔ35 mothers, and partially rescues the X chromosome non-disjunction, impaired double strand break repair, pachytene arrest and delayed karyosome dynamics phenotypes exhibited in jnjΔ35/jnjΔ35 female gonads.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
jnjΔ35
Name Synonyms
Secondary FlyBase IDs
    References (1)