FB2025_01 , released February 20, 2025
Allele: Dmel\Eaat1UAS.Venus
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General Information
Symbol
Dmel\Eaat1UAS.Venus
Species
D. melanogaster
Name
FlyBase ID
FBal0321681
Feature type
allele
Associated gene
Dmel\Eaat1
Associated Insertion(s)
Carried in Construct
P{UAS-Eaat1.WT.Venus}
Key Links
Transgenic product class
cDNA
(Parinejad et al., 2016)
wild_type
(Parinejad et al., 2016)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Parinejad et al., 2016)
wild_type
(Parinejad et al., 2016)
Mutagen
in vitro construct
(Parinejad et al., 2016)
Progenitor genotype
Carried in construct
P{UAS-Eaat1.WT.Venus}
Cytology
Description

UASt regulatory sequences drive expression of the wild-type Eaat1 coding region (derived from the RE20434 cDNA clone) tagged at the C-terminal end with Venus.

(Parinejad et al., 2016)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Eaat1
Tagged with
Venus
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4repo/Eaat1UAS.Venus rescues Eaat1SM2/Eaat1hypo

      (Peng et al., 2019)

      Scer\GAL4repo/Eaat1UAS.Venus rescues Eaat1hypo

      (Peng et al., 2019)

      Scer\GAL4repo.PU/Eaat1UAS.Venus rescues Eaat1SM2

      (Parinejad et al., 2016)
      Partially rescues

      Eaat1UAS.Venus/Scer\GAL4alrm.PD partially rescues Eaat1hypo

      (Peng et al., 2019)

      Scer\GAL4nSyb.PS/Eaat1UAS.Venus partially rescues Eaat1hypo

      (Peng et al., 2019)

      Scer\GAL4tsh-Gal4-1/Eaat1UAS.Venus partially rescues Eaat1hypo

      (Peng et al., 2019)
      Comments

      Constitutive expression of Eaat1UAS.Venus under the control of Scer\GAL4repo rescues the locomotor defects of Eaat1hypo homozygous larvae; the same rescue is obtained by conditional expression only during L1-L3 , L2-L3, L3, or mL3 (temporal regulation of expression is under the control of Gal80[ts] and temperature shift from 18[o]C to 29[o]C).

      (Peng et al., 2019)

      Expression of Eaat1Scer\UAS.T:Avic\GFP-YFP.Venus under the control of Scer\GAL4repo.PU rescues the loss of peristaltic contractions seen in Eaat1SM2/Eaat1SM2 mutant larvae.

      (Parinejad et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Eaat1Scer\UAS.T:Avic\GFP-YFP.Venus
      Eaat1UAS.Venus
      Name Synonyms
      Secondary FlyBase IDs
        References (2)