FlyBase Allele Report: Hsap\BCL2L13[UAS.cNa]

General Information

Symbol

Hsap\BCL2L13^UAS.cNa

Species

H. sapiens

Name

Saccharomyces cerevisiae UAS construct a of Nakazawa

FlyBase ID

FBal0321721

Feature type

allele

Associated gene

Hsap\BCL2L13

Associated Insertion(s)

Carried in Construct

P{UAS-hBCL2L13.N}

Key Links

Transgenic product class

wild_type

(Nakazawa et al., 2016)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Nakazawa et al., 2016)

Mutagen

in vitro construct

(Nakazawa et al., 2016)

Progenitor genotype

Carried in construct

P{UAS-hBCL2L13.N}

Cytology

Description

UAS regulatory sequences drive expression of Hsap\BCL2L13.

(Nakazawa et al., 2016)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsap\BCL2L13

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape | P-stage, with Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

abnormal eye color, with Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

increased cell death | third instar larval stage, with Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

lethal - all die during embryonic stage, with Scer\GAL4^en.PU

(Nakazawa et al., 2016)

visible, with Scer\GAL4^dpp.PU

(Nakazawa et al., 2016)

visible, with Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

visible, with Scer\GAL4^pnr.PU

(Nakazawa et al., 2016)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Hsap\BCL2L13^Scer\UAS.cNa driven along the anterior-posterior compartment boundary by Scer\GAL4^dpp.PU induces morphological changes in the L3 wing vein, and the L3-L4 area is significantly smaller than in controls. Expression of Hsap\BCL2L13^Scer\UAS.cNa driven in the dorsal mesothorax by Scer\GAL4^pnr.PU results in a split thorax phenotype. Expression of Hsap\BCL2L13^Scer\UAS.cNa driven in the posterior compartment of each segment of the embryo by Scer\GAL4^en.PU induces embryonic lethality.

Expression of Hsap\BCL2L13^Scer\UAS.cNa driven in the eye by Scer\GAL4^GMR.PS induces a rough eye phenotype (reduced eye size and loss of ommatidia, bristles and pigmentation), compared to controls; expression induces significant activation of caspase in the morphogenetic furrow of eye imaginal discs in third instar larvae, unlike in controls. The shape of cone and pigment cells are markedly altered (four cone cells per ommatidium and surrounding pigment cells are hardly distinguishable) in the Scer\GAL4^GMR.PS>Hsap\BCL2L13^Scer\UAS.cNa pupal retina 42hr after puparium formation; there are no significant signs of apoptosis being induced in the pupal retinae, similar to controls. Expression of Hsap\BCL2L13^Scer\UAS.cNa driven by Scer\GAL4^GMR.PS does not affect differentiation of photoreceptor cells in the eye discs of third instar larvae, but the differentiation of cone and pigment cells is affected in pupal retinae.

(Nakazawa et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has visible phenotype, non-enhanceable by Debcl[+]/Debcl^E26

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has visible phenotype, non-enhanceable by Debcl[+]/Debcl^W105

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has visible phenotype, non-enhanceable by Buffy[+]/Buffy^H37

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has visible phenotype, non-enhanceable by Buffy[+]/Debcl[+]/Debcl^E26/Buffy^H37

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has visible phenotype, non-enhanceable by Buffy[+]/Debcl[+]/Buffy^H37/Debcl^W105

(Nakazawa et al., 2016)

Suppressed by

Statement

Reference

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has abnormal eye color phenotype, suppressible by BacA\p35^UAS.cUa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has abnormal eye color phenotype, suppressible by Diap1^UAS.cUa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has abnormal eye color phenotype, suppressible by Diap2^GMR.PH

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has visible phenotype, suppressible | partially by BacA\p35^UAS.cUa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has visible phenotype, suppressible | partially by Diap1^UAS.cUa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has visible phenotype, suppressible | partially by Diap2^GMR.PH

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has abnormal cell shape | P-stage phenotype, suppressible | partially by BacA\p35^UAS.cUa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has abnormal eye color phenotype, suppressible by sesB^G0247/Ant2^G0247/sesB[+]/Ant2[+]

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has abnormal eye color phenotype, suppressible by sesB^org/sesB[+]

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has abnormal eye color phenotype, suppressible by Atg8a[+]/Atg8a^EP362

(Nakazawa et al., 2016)

NOT suppressed by

Statement

Reference

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has abnormal eye color phenotype, non-suppressible by schlank^G0061

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has visible phenotype, non-suppressible by Debcl[+]/Debcl^E26

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has visible phenotype, non-suppressible by Debcl[+]/Debcl^W105

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has visible phenotype, non-suppressible by Buffy[+]/Buffy^H37

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has visible phenotype, non-suppressible by Buffy[+]/Debcl[+]/Debcl^E26/Buffy^H37

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has visible phenotype, non-suppressible by Buffy[+]/Debcl[+]/Buffy^H37/Debcl^W105

(Nakazawa et al., 2016)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has eye phenotype, non-enhanceable by Debcl[+]/Debcl^E26

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has eye phenotype, non-enhanceable by Debcl[+]/Debcl^W105

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has eye phenotype, non-enhanceable by Buffy[+]/Buffy^H37

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has eye phenotype, non-enhanceable by Buffy[+]/Debcl[+]/Debcl^E26/Buffy^H37

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has eye phenotype, non-enhanceable by Buffy[+]/Debcl[+]/Buffy^H37/Debcl^W105

(Nakazawa et al., 2016)

Suppressed by

Statement

Reference

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has eye phenotype, suppressible | partially by BacA\p35^UAS.cUa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has interommatidial bristle phenotype, suppressible | partially by BacA\p35^UAS.cUa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has ommatidium phenotype, suppressible | partially by BacA\p35^UAS.cUa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has eye phenotype, suppressible | partially by Diap1^UAS.cUa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has interommatidial bristle phenotype, suppressible | partially by Diap1^UAS.cUa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has ommatidium phenotype, suppressible | partially by Diap1^UAS.cUa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has eye phenotype, suppressible | partially by Diap2^GMR.PH

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has interommatidial bristle phenotype, suppressible | partially by Diap2^GMR.PH

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has ommatidium phenotype, suppressible | partially by Diap2^GMR.PH

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has cone cell | P-stage phenotype, suppressible | partially by BacA\p35^UAS.cUa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has pigment cell | P-stage phenotype, suppressible | partially by BacA\p35^UAS.cUa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has interommatidial bristle phenotype, suppressible | partially by Atg8a[+]/Atg8a^EP362

(Nakazawa et al., 2016)

NOT suppressed by

Statement

Reference

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has eye phenotype, non-suppressible by Debcl[+]/Debcl^E26

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has eye phenotype, non-suppressible by Debcl[+]/Debcl^W105

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has eye phenotype, non-suppressible by Buffy[+]/Buffy^H37

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has eye phenotype, non-suppressible by Buffy[+]/Debcl[+]/Debcl^E26/Buffy^H37

(Nakazawa et al., 2016)

Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS has eye phenotype, non-suppressible by Buffy[+]/Debcl[+]/Buffy^H37/Debcl^W105

(Nakazawa et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of BacA\p35^Scer\UAS.cUa, Diap1^Scer\UAS.cUa or Diap2^GMR.PH suppresses the reduction of eye pigments and weakly suppresses the abnormal formation of ommatidia and bristles and reduced eye size seen in Scer\GAL4^GMR.PS>Hsap\BCL2L13^Scer\UAS.cNa flies. Co-expression of BacA\p35^Scer\UAS.cUa partially suppresses aberrant morphology of cone and pigment cells in the Scer\GAL4^GMR.PS>Hsap\BCL2L13^Scer\UAS.cNa pupal retina 42hr after puparium formation.

The rough eye phenotype induced by expression of Hsap\BCL2L13^Scer\UAS.cNa driven by Scer\GAL4^GMR.PS is not significantly enhanced or suppressed by Debcl^E26/+, Debcl^W105/+, Debcl^E26 Buffy^H37/+, Debcl^W105 Buffy^H37/+ or Buffy^H37/+ mutations.

The loss of pigment in the eye phenotype induced by expression of Hsap\BCL2L13^Scer\UAS.cNa driven by Scer\GAL4^GMR.PS is significantly suppressed by sesB^org/+ or Ant2^G0247 sesB^G0247/+; schlank^G0061 did not suppress the loss of pigment phenotype. Atg8a^EP362/+ suppresses the loss of pigmentation and partially suppresses loss of bristles in flies with expression of Hsap\BCL2L13^Scer\UAS.cNa driven by Scer\GAL4^GMR.PS.

(Nakazawa et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hsap\BCL2L13^Scer\UAS.cNa

Hsap\BCL2L13^UAS.cNa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Nakazawa

Secondary FlyBase IDs

References (1)

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