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General Information
Symbol
Hsap\BCL2L13UAS.cNa
Species
H. sapiens
Name
Saccharomyces cerevisiae UAS construct a of Nakazawa
FlyBase ID
FBal0321721
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of Hsap\BCL2L13.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Hsap\BCL2L13Scer\UAS.cNa driven along the anterior-posterior compartment boundary by Scer\GAL4dpp.PU induces morphological changes in the L3 wing vein, and the L3-L4 area is significantly smaller than in controls. Expression of Hsap\BCL2L13Scer\UAS.cNa driven in the dorsal mesothorax by Scer\GAL4pnr.PU results in a split thorax phenotype. Expression of Hsap\BCL2L13Scer\UAS.cNa driven in the posterior compartment of each segment of the embryo by Scer\GAL4en.PU induces embryonic lethality.

Expression of Hsap\BCL2L13Scer\UAS.cNa driven in the eye by Scer\GAL4GMR.PS induces a rough eye phenotype (reduced eye size and loss of ommatidia, bristles and pigmentation), compared to controls; expression induces significant activation of caspase in the morphogenetic furrow of eye imaginal discs in third instar larvae, unlike in controls. The shape of cone and pigment cells are markedly altered (four cone cells per ommatidium and surrounding pigment cells are hardly distinguishable) in the Scer\GAL4GMR.PS>Hsap\BCL2L13Scer\UAS.cNa pupal retina 42hr after puparium formation; there are no significant signs of apoptosis being induced in the pupal retinae, similar to controls. Expression of Hsap\BCL2L13Scer\UAS.cNa driven by Scer\GAL4GMR.PS does not affect differentiation of photoreceptor cells in the eye discs of third instar larvae, but the differentiation of cone and pigment cells is affected in pupal retinae.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement
Reference

Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS has visible phenotype, non-enhanceable by Debcl[+]/DebclE26

Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS has visible phenotype, non-enhanceable by Debcl[+]/DebclW105

Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS has visible phenotype, non-enhanceable by Buffy[+]/BuffyH37

Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS has visible phenotype, non-enhanceable by Buffy[+]/Debcl[+]/DebclE26/BuffyH37

Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS has visible phenotype, non-enhanceable by Buffy[+]/Debcl[+]/BuffyH37/DebclW105

Suppressed by
NOT suppressed by
Statement
Reference

Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS has visible phenotype, non-suppressible by Debcl[+]/DebclE26

Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS has visible phenotype, non-suppressible by Debcl[+]/DebclW105

Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS has visible phenotype, non-suppressible by Buffy[+]/BuffyH37

Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS has visible phenotype, non-suppressible by Buffy[+]/Debcl[+]/DebclE26/BuffyH37

Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS has visible phenotype, non-suppressible by Buffy[+]/Debcl[+]/BuffyH37/DebclW105

Phenotype Manifest In
NOT Enhanced by
Statement
Reference

Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS has eye phenotype, non-enhanceable by Debcl[+]/DebclE26

Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS has eye phenotype, non-enhanceable by Debcl[+]/DebclW105

Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS has eye phenotype, non-enhanceable by Buffy[+]/BuffyH37

Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS has eye phenotype, non-enhanceable by Buffy[+]/Debcl[+]/DebclE26/BuffyH37

Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS has eye phenotype, non-enhanceable by Buffy[+]/Debcl[+]/BuffyH37/DebclW105

Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference

Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS has eye phenotype, non-suppressible by Debcl[+]/DebclE26

Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS has eye phenotype, non-suppressible by Debcl[+]/DebclW105

Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS has eye phenotype, non-suppressible by Buffy[+]/BuffyH37

Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS has eye phenotype, non-suppressible by Buffy[+]/Debcl[+]/DebclE26/BuffyH37

Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS has eye phenotype, non-suppressible by Buffy[+]/Debcl[+]/BuffyH37/DebclW105

Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Co-expression of BacA\p35Scer\UAS.cUa, Diap1Scer\UAS.cUa or Diap2GMR.PH suppresses the reduction of eye pigments and weakly suppresses the abnormal formation of ommatidia and bristles and reduced eye size seen in Scer\GAL4GMR.PS>Hsap\BCL2L13Scer\UAS.cNa flies. Co-expression of BacA\p35Scer\UAS.cUa partially suppresses aberrant morphology of cone and pigment cells in the Scer\GAL4GMR.PS>Hsap\BCL2L13Scer\UAS.cNa pupal retina 42hr after puparium formation.

The rough eye phenotype induced by expression of Hsap\BCL2L13Scer\UAS.cNa driven by Scer\GAL4GMR.PS is not significantly enhanced or suppressed by DebclE26/+, DebclW105/+, DebclE26 BuffyH37/+, DebclW105 BuffyH37/+ or BuffyH37/+ mutations.

The loss of pigment in the eye phenotype induced by expression of Hsap\BCL2L13Scer\UAS.cNa driven by Scer\GAL4GMR.PS is significantly suppressed by sesBorg/+ or Ant2G0247 sesBG0247/+; schlankG0061 did not suppress the loss of pigment phenotype. Atg8aEP362/+ suppresses the loss of pigmentation and partially suppresses loss of bristles in flies with expression of Hsap\BCL2L13Scer\UAS.cNa driven by Scer\GAL4GMR.PS.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Hsap\BCL2L13Scer\UAS.cNa
Hsap\BCL2L13UAS.cNa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Nakazawa
Secondary FlyBase IDs
    References (1)