FB2025_01 , released February 20, 2025
Allele: Dmel\AckUAS.Tag:V5
Open Close
General Information
Symbol
Dmel\AckUAS.Tag:V5
Species
D. melanogaster
Name
FlyBase ID
FBal0321753
Feature type
allele
Associated gene
Dmel\Ack
Associated Insertion(s)
Carried in Construct
P{UAS-Ack.V5}
Key Links
Transgenic product class
cDNA
(Hu et al., 2016)
wild_type
(Hu et al., 2016)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Hu et al., 2016)
wild_type
(Hu et al., 2016)
Mutagen
in vitro construct
(Hu et al., 2016)
Progenitor genotype
Carried in construct
P{UAS-Ack.V5}
Cytology
Description

UASt regulatory sequences drive expression of the Ack GH10777 cDNA. The open reading frame is tagged with Tag:V5.

(Hu et al., 2016)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Ack
Tagged with
Tag:V5
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of AckScer\UAS.T:SV5\V5 under the control of either Scer\GAL4Bx-MS1096 or Scer\GAL4GMR.PU promotes excessive growth resulting in adult wings and eyes (respectively) that are significantly larger than in the wild-type controls.

      (Hu et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Statement
      Reference

      AckUAS.Tag:V5, Scer\GAL4GMR.PU has visible | adult stage phenotype, enhanceable by ykiGD11187, Scer\GAL4GMR.PU

      (Hu et al., 2016)
      Suppressed by
      Enhancer of
      NOT Suppressor of
      Statement
      Reference

      AckUAS.Tag:V5/Scer\GAL4Ubi.PU is a non-suppressor of visible | somatic clone | adult stage phenotype of exe1

      (Hu et al., 2016)
      Other
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      AckUAS.Tag:V5, Scer\GAL4GMR.PU has eye | adult stage phenotype, enhanceable by ykiGD11187, Scer\GAL4GMR.PU

      (Hu et al., 2016)
      Suppressed by
      Statement
      Reference

      AckUAS.Tag:V5, Scer\GAL4Bx-MS1096 has wing blade phenotype, suppressible by ykiGD11187, Scer\GAL4Bx-MS1096

      (Hu et al., 2016)
      Enhancer of
      NOT Enhancer of
      Statement
      Reference

      AckUAS.Tag:V5/Scer\GAL4Ubi.PU is a non-enhancer of eye | adult stage | somatic clone phenotype of exe1

      (Hu et al., 2016)

      AckUAS.Tag:V5/Scer\GAL4Ubi.PU is a non-enhancer of antenna | adult stage | somatic clone phenotype of exe1

      (Hu et al., 2016)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The increased adult wing size characteristic for flies expressing AckScer\UAS.T:SV5\V5 under the control of Scer\GAL4Bx-MS1096 is completely suppressed by coexpression of ykiGD11187 : the resulting wings are severely undergrown and malformed compared to wild-type and their size is not significantly different from when ykiGD11187 is expressed on its own.

      Expression of AckScer\UAS.T:SV5\V5 under the control of Scer\GAL4Ubi.PU in either the Mer4 or the kibra4 homozygous somatic clones (induced using the eyFLP method) promotes tissue overgrowth in the adult eye.

      The eye overgrowth observed in adult flies expressing either ykiScer\UAS.cZa or ykiS168A.Scer\UAS.cRa.T:Hsap\MYC under the control of Scer\GAL4GMR.PU is exacerbated further by co-expression of AckScer\UAS.T:SV5\V5 (which on its own also promotes eye growth).

      Simultaneous expression of AckScer\UAS.T:SV5\V5, which on its own leads to wing overgrowth, and any of the following: exScer\UAS.cUa, ex5YA.Scer\UAS, exY227A.Scer\UAS, exY423A.Scer\UAS, exY679A.Scer\UAS, exY766A.Scer\UAS, exY1103A.Scer\UAS which each on its own leads to very small and malformed wings, under the control of Scer\GAL4Bx-MS1096 results in wings of intermediate size but smaller than in wild-type controls. In the case of ex5YA.Scer\UAS co-expression the wings are only slightly bigger than with ex5YA.Scer\UAS expressed alone.

      The overgrowth phenotype observed in adult eye and antennae containing exe1 homozygous mutant somatic clones (induced by the eyFLP method) is not changed when the clones also express AckScer\UAS.T:SV5\V5 under the control of Scer\GAL4Ubi.PU. However, expression of AckScer\UAS.T:SV5\V5 (but not AckKD.Scer\UAS.T:SV5\V5) significantly enhances the overgrowth phenotype induced by hpoBF33 clones.

      (Hu et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      AckScer\UAS.T:SV5\V5
      AckUAS.Tag:V5
      Name Synonyms
      Secondary FlyBase IDs
        References (1)