amorphic allele - molecular evidence
lncRNA region removed by targeted deletion.
The entire lncRNA gene has been knocked out and replaced with an attP site followed by an FRT-flanked Disc\RFP3xP3.cUa fluorescent marker.
CR43862KO homozygote males show reduced fertility compared to wild-type controls and display defects in spermatid morphogenesis (mutant testes contain sperm cells that are scattered or curled).
lncRNA:CR43862KO is rescued by lncRNA:CR43862+tWa
The late spermatogenesis defects observed in CR43862KO homozygous males are rescued by lncRNA restoration with CR43862+tWa both in cis (in situ rescue) and in trans.