FB2020_06, released Dec 22, 2020

Allele: Dmel\MESR479

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General Information
Symbol
Dmel\MESR479
Species
D. melanogaster
Name
FlyBase ID
FBal0322739
Feature type
allele
Associated gene
Dmel\MESR4
Associated Insertion(s)
Carried in Construct
Genomic Maps
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Wissel et al., 2016)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
2R:17,549,078..17,549,093 [+]
Comment:

Deletion of 15 bases (GCAGATAATATGATG) including the ATG start codon.

(Wissel et al., 2016)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Cytology
      Nature of the lesion
      Statement
      Reference

      15bp deletion that removes the ATG start codon

      (Wissel et al., 2016)
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      MESR479 homozygous mutants are pupal lethal. Expression of Avic\GFPdsRNA.shRNA.Scer\UAS.1 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in MESR479/MESR4V5.miGFPi transheterozygotes to knock down MESR4 using the miGFPi method (tagging a gene with a minimal GFP tag and then expressing RNAi against the tag) leads to small ovaries and germline differentiation defects (same as in MESR4V5.miGFPi homozygotes).

      (Wissel et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      MESR479
      (Wissel et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)