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General Information
Symbol
Dmel\lolaV5.miGFPi.PB
Species
D. melanogaster
Name
FlyBase ID
FBal0322746
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Mutagen
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

A cassette comprised of the Tag:V5 epitope plus the Tag:miGFPi RNAi-effector sequence has been inserted in frame at the C-terminal end of the open reading frame of the lola-PB isoform. The position of the cassette allows for specific knockdown of the lola-PB isoform using an RNAi reagent that targets EGFP.

Insertion components
TI{TI}lolaV5.miGFPi.PB
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Avic\GFPdsRNA.shRNA.Scer\UAS.1 under the control of Scer\GAL4wor.PA in lolaV5.miGFPi.PB homozygotes to knock-down only the lola-PB isoform using the miGFPi method (tagging a gene with a minimal GFP tag and then expressing RNAi against the tag) does not cause overproliferation of neural stem cells in the larval central brain. Similarly, expression of Avic\GFPdsRNA.shRNA.Scer\UAS.1 in lolaV5.miGFPi.all/lolaV5.miGFPi.PB larvae (=effectively knocking down lola-PB only) also does not cause neuroblast overproliferation but when both lola-PD and lola-PB isoforms are knocked-down simultaneously in lolaV5.miGFPi.all/lolaV5.miGFPi.PD,lolaHA.miGFPi.PD larvae, neuroblast overproliferation is observed.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
lolaV5.miGFPi.PB
Name Synonyms
Secondary FlyBase IDs
    References (1)