FB2021_01, released Feb 18, 2021

Allele: Dmel\lolaV5.miGFPi.PB

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General Information
Symbol
Dmel\lolaV5.miGFPi.PB
Species
D. melanogaster
Name
FlyBase ID
FBal0322746
Feature type
allele
Associated gene
Dmel\lola
Associated Insertion(s)
TI{TI}lolaV5.miGFPi.PB
Carried in Construct
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Wissel et al., 2016)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Cytology
      Nature of the lesion
      Statement
      Reference

      A cassette comprised of the Tag:V5 epitope plus the Tag:miGFPi RNAi-effector sequence has been inserted in frame at the C-terminal end of the open reading frame of the lola-PB isoform. The position of the cassette allows for specific knockdown of the lola-PB isoform using an RNAi reagent that targets EGFP.

      (Wissel et al., 2016)
      Insertion components
      TI{TI}lolaV5.miGFPi.PB
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Avic\GFPdsRNA.shRNA.Scer\UAS.1 under the control of Scer\GAL4wor.PA in lolaV5.miGFPi.PB homozygotes to knock-down only the lola-PB isoform using the miGFPi method (tagging a gene with a minimal GFP tag and then expressing RNAi against the tag) does not cause overproliferation of neural stem cells in the larval central brain. Similarly, expression of Avic\GFPdsRNA.shRNA.Scer\UAS.1 in lolaV5.miGFPi.all/lolaV5.miGFPi.PB larvae (=effectively knocking down lola-PB only) also does not cause neuroblast overproliferation but when both lola-PD and lola-PB isoforms are knocked-down simultaneously in lolaV5.miGFPi.all/lolaV5.miGFPi.PD,lolaHA.miGFPi.PD larvae, neuroblast overproliferation is observed.

      (Wissel et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      lolaV5.miGFPi.PB
      Name Synonyms
      Secondary FlyBase IDs
        References (1)