FB2025_05 , released December 11, 2025
Allele: Dmel\sit1D
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General Information
Symbol
Dmel\sit1D
Species
D. melanogaster
Name
FlyBase ID
FBal0322763
Feature type
allele
Associated gene
Dmel\sit
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Danielsen et al., 2016)
Mutagen
CRISPR/Cas9
(Danielsen et al., 2016)
Progenitor genotype
Cytology
Description

A 1325bp deletion encompassing the entire coding sequence of sit except the last few nucleotides.

(Danielsen et al., 2016)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
delins
3R:22,559,019..22,560,345 [-]
Inserted_sequence:

AAGGCTTGTAAGCTTG

Comment:

Site of CRISPR/Cas9 deletion of all but the last few bases of the sit coding sequence. 1327 bases were deleted and 16 bases (AAGGCTTGTAAGCTTG) were inserted at the site of the deletion.

(Danielsen et al., 2016)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      sit1D homozygous as well as sit1D/sit2C transheterozygous mutants are developmentally lethal with majority of the animals dying during larval stages and display increased number of lipid droplets in the prothoracic gland cells but not in the fat body cells.

      (Danielsen et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      sit1D
      (Danielsen et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)