FB2025_01 , released February 20, 2025
Allele: Dmel\FIG4RNAi.RNAi.cBa
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General Information
Symbol
Dmel\FIG4RNAi.RNAi.cBa
Species
D. melanogaster
Name
FlyBase ID
FBal0322831
Feature type
allele
Associated gene
Dmel\FIG4
Associated Insertion(s)
Carried in Construct
P{UAS-FIG4.RNAi.B}
Key Links
Transgenic product class
RNAi_reagent
(Bharadwaj et al., 2016)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Bharadwaj et al., 2016)
Mutagen
in vitro construct
(Bharadwaj et al., 2016)
Progenitor genotype
Carried in construct
P{UAS-FIG4.RNAi.B}
Cytology
Description

UAS regulatory sequences drive expression of two miRNAs directed against FIG4 (the miRNA sequences are in tandem, and each target a different region of FIG4).

(Bharadwaj et al., 2016)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
FIG4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of FIG4dsRNA.miRNA.Scer\UAS under the control of Scer\GAL4Mef2.PU leads to lysosome expansion phenotype in the somatic muscles of third instar larvae.

      (Bharadwaj et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The lysosome expansion phenotype observed in third instar larvae expressing FIG4dsRNA.miRNA.Scer\UAS under the control of Scer\GAL4Mef2.PU suppressed by co-expression of any of the following: Rab7dsRNA.Scer\UAS.cUa, Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP or ltdsRNA.Scer\UAS.cUa and can be further enhanced by co-expression of Rab7Q67L.Scer\UAS.P\T.T:Avic\GFP-YFP.

      (Bharadwaj et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      FIG4RNAi.RNAi.UAS
      FIG4RNAi.RNAi.cBa
      FIG4dsRNA.miRNA.Scer\UAS
      FIG4dsRNA.miRNA.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (2)