FlyBase Allele Report: Dmel\ddbt[Z4344]

General Information

Symbol

Dmel\ddbt^Z4344

Species

D. melanogaster

Name

FlyBase ID

FBal0322936

Feature type

allele

Associated gene

Dmel\ddbt

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Yamaki et al., 2016)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q48term.

(Yamaki et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:317,349..317,349 [+]

Nucleotide change:

C317349T

Reported nucleotide change:

C?T

Amino acid change:

Q48term | ddbt-PB; Q48term | ddbt-PC; Q48term | ddbt-PD

Reported amino acid change:

Q48term

(Yamaki et al., 2016)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | paternal effect

(Yamaki et al., 2016)

abnormal mitotic cell cycle | embryonic stage | paternal effect

(Yamaki et al., 2016)

lethal - all die during embryonic stage | paternal effect

(Yamaki et al., 2016)

male sterile (with Df(3L)7C)

(Yamaki et al., 2016)

male sterile | recessive

(Yamaki et al., 2016)

Phenotype Manifest In

chromosome | embryonic stage | paternal effect

(Yamaki et al., 2016)

spermatid | adult stage

(Yamaki et al., 2016)

telomere cap complex | adult stage | male

(Yamaki et al., 2016)

Detailed Description

Statement

Reference

ddbt^Z4344/Df(3L)7C and ddbt^Z4344 homozygous males are sterile but display no sperm morphological defects.

ddbt^Z4344 homozygote males fail to retain the telomere-capping complexes through spermiogenesis: components of the complex are lost between early and late canoe stage.

Early embryos from ddbt^Z4344 homozygous fathers have abnormal karyotype with variably sized clumps of chromatin and frequent chromosome fragmentations. Majority of these embryos are in prometa- or metaphase and frequently display abnormal mitotic figures. They also show developmental delay as well as defects in paternal chromosome segregation and chromatin bridging in the earliest cell cycles. Most of the embryos of ddbt^Z4344 fathers arrest at cycle 3 or shortly after (never finish cycle 6) and the abnormalities they display include loss of nuclear synchrony, hyper-condensation, variation in ploidy and chromatin chromosome fragmentation with the defects becoming more frequent with age. Minority of the embryos progress beyond the early cycles and these show haploid or haploid mosaic karyotypes, suggesting that the paternal chromosomes are subjected to continued elimination.

(Yamaki et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ddbt^Z4344 has abnormal developmental rate | paternal effect phenotype, suppressible | maternal effect | partially by lok^p6

(Yamaki et al., 2016)

NOT suppressed by

Statement

Reference

ddbt^Z4344 has abnormal developmental rate | paternal effect phenotype, non-suppressible | maternal effect | partially by lok^p6

(Yamaki et al., 2016)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The developmental delay and arrest at cycle 3 characteristic for embryos from ddbt^Z4344 fathers is partially rescued in embryos from ddbt^Z4344 fathers and lok^p6 mothers - these embryos are allowed to progress past cycle 3 despite the number of chromosomal abnormalities they display (lagging chromosomes, anaphase bridges and nuclei with variable chromosome content). No such effect is observed in embryos from ddbt^Z4344 fathers and grp^fs1 mothers.

(Yamaki et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

ddbt^Z4344/Df(3L)7C is rescued by ddbt^+t3.8

(Yamaki et al., 2016)

ddbt^Z4344 is rescued by ddbt^mCherry

(Yamaki et al., 2016)

Partially rescued by

ddbt^Z4344/Df(3L)7C is partially rescued by ddbt^EGFP

(Yamaki et al., 2016)

Not rescued by

ddbt^Z4344 is not rescued by ddbt^{NFLR-Mst35Ba.EGFP}

(Yamaki et al., 2016)

ddbt^Z4344 is not rescued by ddbt^{NFLR-Mst77F.EGFP}

(Yamaki et al., 2016)

ddbt^Z4344 is not rescued by ddbt^R31D.EGFP

(Yamaki et al., 2016)

Comments

The male sterility of ddbt^Z4344/Df(3L)7C or ddbt^Z4344 homozygous flies is rescued to a high degree by combination with ddbt^{T:Avic\GFP-EGFP} or ddbt^{T:Disc\RFP-mCherry} or ddbt^+t3.8.

The male sterility of ddbt^Z4344 homozygous flies cannot be rescued by combination with any of the following: ddbt[NFLR-Mst35Ba. T:Avic \GFP-EGFP], ddbt^{NFLR-Mst77F.T:Avic\GFP-EGFP} or ddbt[R31D. T:Avic \GFP-EGFP]

(Yamaki et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

ddbt^Z4344

(Yamaki et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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