Expression of Hsap\MAPTScer\UAS.T:Avic\GFP under the control of Scer\GAL4shot-OK307 in the htt98E2 homozygous mutant background induces prominent collapse of the thorax due to severe loss of the muscles below, these phenotypes are not observed in either the Hsap\MAPTScer\UAS.T:Avic\GFP expressing flies or the htt98E2 mutants alone and can be rescued by combination with a single copy of htt+m22.7 or by co-expression of Hsap\HTT16Q.FL.Scer\UAS. Expression of Hsap\MAPTScer\UAS.T:Avic\GFP in either htt98E2 or Atg8ad4 heterozygous background does not produce any obvious phenotype in the adult thorax. However, when Hsap\MAPTScer\UAS.T:Avic\GFP is expressed in either htt98E2/+;Atg8ad4/+ double heterozygotes or Atg8ad4 homozygotes nearly all the adult flies have collapsed thoraces and display loss of skeletal thoracic muscles. Expression of Hsap\MAPTScer\UAS.T:Avic\GFP in Atg1Δ3D/+ heterozygous background leads to moderate thoracic collapse and loss of skeletal muscles, this phenotype is strongly enhanced by combination with a single copy of htt98E2.
Hsap\MAPTScer\UAS.T:Avic\GFP expression in adults heterozygous for any of the following alleles: Atg13Δ81, Atg7d77 or ref(2)Pc03993 has no phenotype, unless the animals are also heterozygous for htt98E2, in which case a severe loss of thoracic somatic muscles is observed.
Co-expression of either of the temperature sensitive Prosβ61.B.Scer\UAS or Prosβ21.Scer\UAS alleles together with Hsap\MAPTScer\UAS.T:Avic\GFP under the control of Scer\GAL4shot-OK307 has no adverse effect on the morphology and structure of the adult thorax or the thoracic somatic muscles below at either the non-permissive (28[o]C) or the permissive (22[o]C) temperatures.
The age-dependent loss of climbing ability as well as the reduced lifespan characteristic for htt98E2 homozygous adults is further worsened by expression of Hsap\MAPTScer\UAS.T:Avic\GFP under the control of Scer\GAL4shot-OK307 in the mutant background. This worsened phenotype can in turn be fully rescued by combination with a single copy of htt+m22.7 and partially rescued by co-expression of Hsap\HTT16Q.FL.Scer\UAS.