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General Information
Symbol
Hsap\MAPTUAS.GFP
Species
H. sapiens
Name
FlyBase ID
FBal0323607
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of full-length Hsap\MAPT tagged at the C-terminal end with GFP.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expressing Hsap\MAPTUAS.GFP under the control of Scer\GAL4GMR.PU causes a severe rough eye phenotype with necrotic scars.

Expression of Hsap\MAPTScer\UAS.T:Avic\GFP under the control of Scer\GAL4shot-OK307 has no adverse effect on the adult thorax morphology and structure, nor does it affect the thoracic skeletal muscles.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
Phenotype Manifest In
Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Expression of Hsap\MAPTScer\UAS.T:Avic\GFP under the control of Scer\GAL4shot-OK307 in the htt98E2 homozygous mutant background induces prominent collapse of the thorax due to severe loss of the muscles below, these phenotypes are not observed in either the Hsap\MAPTScer\UAS.T:Avic\GFP expressing flies or the htt98E2 mutants alone and can be rescued by combination with a single copy of htt+m22.7 or by co-expression of Hsap\HTT16Q.FL.Scer\UAS. Expression of Hsap\MAPTScer\UAS.T:Avic\GFP in either htt98E2 or Atg8ad4 heterozygous background does not produce any obvious phenotype in the adult thorax. However, when Hsap\MAPTScer\UAS.T:Avic\GFP is expressed in either htt98E2/+;Atg8ad4/+ double heterozygotes or Atg8ad4 homozygotes nearly all the adult flies have collapsed thoraces and display loss of skeletal thoracic muscles. Expression of Hsap\MAPTScer\UAS.T:Avic\GFP in Atg1Δ3D/+ heterozygous background leads to moderate thoracic collapse and loss of skeletal muscles, this phenotype is strongly enhanced by combination with a single copy of htt98E2.

Hsap\MAPTScer\UAS.T:Avic\GFP expression in adults heterozygous for any of the following alleles: Atg13Δ81, Atg7d77 or ref(2)Pc03993 has no phenotype, unless the animals are also heterozygous for htt98E2, in which case a severe loss of thoracic somatic muscles is observed.

Co-expression of either of the temperature sensitive Prosβ61.B.Scer\UAS or Prosβ21.Scer\UAS alleles together with Hsap\MAPTScer\UAS.T:Avic\GFP under the control of Scer\GAL4shot-OK307 has no adverse effect on the morphology and structure of the adult thorax or the thoracic somatic muscles below at either the non-permissive (28[o]C) or the permissive (22[o]C) temperatures.

The age-dependent loss of climbing ability as well as the reduced lifespan characteristic for htt98E2 homozygous adults is further worsened by expression of Hsap\MAPTScer\UAS.T:Avic\GFP under the control of Scer\GAL4shot-OK307 in the mutant background. This worsened phenotype can in turn be fully rescued by combination with a single copy of htt+m22.7 and partially rescued by co-expression of Hsap\HTT16Q.FL.Scer\UAS.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Hsap\MAPTScer\UAS.T:Avic\GFP
Hsap\MAPTUAS.GFP
Name Synonyms
Secondary FlyBase IDs
    References (2)