FlyBase Allele Report: Dmel\Mettl3[Δcat]

General Information

Symbol

Dmel\Mettl3^Δcat

Species

D. melanogaster

Name

FlyBase ID

FBal0324795

Feature type

allele

Associated gene

Dmel\Mettl3

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(Lence et al., 2016)

Progenitor genotype

Cytology

Description

A deletion of 569 bp (607-1,175 bp in the genome region chr3R:24032157..24034257 , genome assembly BDGP release 6), removing the C-terminal part of Ime4 containing the catalytic domain.

(Lence et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage

(Lence et al., 2016)

abnormal neuroanatomy | adult stage (with Mettl3^null)

(Kan et al., 2021)

decreased efficacy of short-term memory | adult stage (with Mettl3^null)

(Kan et al., 2021)

decreased efficacy of short-term memory | adult stage | progressive (with Df(3R)Exel6197)

(Kan et al., 2021)

partially lethal - majority die

(Rockwell and Hongay, 2020)

short lived

(Lence et al., 2016)

short lived (with Mettl3^null)

(Kan et al., 2021)

viable

(Lence et al., 2016)

viable (with Mettl3^null)

(Lence et al., 2016)

visible (with Mettl3^null)

(Lence et al., 2016)

Phenotype Manifest In

medial lobe of adult mushroom body (with Mettl3^null)

(Kan et al., 2021)

wing

(Lence et al., 2016)

wing (with Mettl3^null)

(Lence et al., 2016)

Detailed Description

Statement

Reference

Ime4^Δcat/Ime4^Δcat flies have a reduced lifespan, show wing posture defects, lower levels of locomotor activity and significantly reduced walking speed, as compared to controls.

Ime4^null/Ime4^Δcat flies have a mild wing posture defect, as compared to controls.

(Lence et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Mettl3^Δcat/Mettl3^null is rescued by Mettl3^{UAS.cLa.Tag:HA}/Scer\GAL4^elav.PLu

(Kan et al., 2021)

Mettl3^Δcat/Mettl3^null is rescued by Scer\GAL4^Mef2.247/Mettl3^{UAS.cLa.Tag:HA}

(Kan et al., 2021)

Mettl3^Δcat is rescued by Scer\GAL4^elav-C155/Mettl3^{UAS.cLa.Tag:HA}

(Lence et al., 2016)

Mettl3^Δcat is rescued by Mettl3^{UAS.cLa.Tag:HA}/Scer\GAL4^Tub.PU

(Lence et al., 2016)

Not rescued by

Mettl3^Δcat is not rescued by Scer\GAL4^how-24B/Mettl3^{UAS.cLa.Tag:HA}

(Lence et al., 2016)

Comments

Expression of Ime4^{Scer\UAS.cLa.T:Ivir\HA1} under the control of Scer\GAL4^elav-C155 or Scer\GAL4^tub.PU, but not Scer\GAL4^how-24B, rescues the wing posture defects, activity levels, and reduced walking speed seen in Ime4^Δcat/Ime4^Δcat flies.

(Lence et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Ime4^Δcat

(Lence et al., 2016)

Ime4ΔCat

(Rockwell and Hongay, 2020)

Mettl3^Δcat

(Perlegos et al., 2022, Kan et al., 2021, Worpenberg et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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