FB2025_01 , released February 20, 2025
Allele: Dmel\ringerUAS.cMa
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General Information
Symbol
Dmel\ringerUAS.cMa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Mino
FlyBase ID
FBal0324984
Feature type
allele
Associated gene
Dmel\ringer
Associated Insertion(s)
Carried in Construct
P{UAS-ringer.M}
Also Known As
UAS-ringer
Key Links
Transgenic product class
cDNA
(Mino et al., 2016)
wild_type
(Mino et al., 2016)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Mino et al., 2016)
wild_type
(Mino et al., 2016)
Mutagen
in vitro construct
(Mino et al., 2016)
Progenitor genotype
Carried in construct
P{UAS-ringer.M}
Cytology
Description

UASt regulates expression of 1.2 Kb of ringer sequence, including 150 bp of the 5'UTR, derived from the RE39465 cDNA.

(Mino et al., 2016)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
ringer
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Ectopic expression of ringerScer\UAS.cMa under the control of Scer\GAL4eve.PU leads to defects in placement of embryonic eve-positive neurons (the phenotype is strongest in RP2 neurons) as well as disruption of axonal bundles. Expression under the Scer\GAL4Act5C.PU driver results in axonal stalling in embryos.

      Cultured neurons (primary cultures from larval brains) expressing ringerScer\UAS.cMa under the control of Scer\GAL4elav-C155 show axonal extension defects and tend to have much shorter axons than control wild-type neurons.

      (Mino et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Scer\GAL4elav-C155/ringerUAS.cMa partially rescues ringer915

      (Mino et al., 2016)

      Scer\GAL4eve.PU/ringerUAS.cMa partially rescues ringer915

      (Mino et al., 2016)
      Comments

      The axonal targeting defects and eve-positive neurons placement errors characteristic for ringer915 homozygous mutant embryos are partially rescued by expression of ringerScer\UAS.cMa under the control of the Scer\GAL4elav-C155 or the Scer\GAL4eve.PU driver, respectively. However, the axonal extension defects observed in cultured ringer915 neurons (primary cultures from larval brains) is not significantly rescued by Scer\GAL4elav-C155-driven expression of ringerScer\UAS.cMa.

      (Mino et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      ringerScer\UAS.cMa
      ringerUAS.cMa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Mino
      Secondary FlyBase IDs
        References (3)