FB2025_01 , released February 20, 2025
Allele: Hsap\TBX2UAS.Tag:FLAG
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General Information
Symbol
Hsap\TBX2UAS.Tag:FLAG
Species
H. sapiens
Name
FlyBase ID
FBal0325058
Feature type
allele
Associated gene
Hsap\TBX2
Associated Insertion(s)
Carried in Construct
M{UAS-TBX2.Flag}
Key Links
Transgenic product class
cDNA
(Shen et al., 2014)
wild_type
(Shen et al., 2014)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Shen et al., 2014)
wild_type
(Shen et al., 2014)
Mutagen
in vitro construct
(Shen et al., 2014)
Progenitor genotype
Carried in construct
M{UAS-TBX2.Flag}
Cytology
Description

UAS regulatory sequences drive expression of a Hsap\TBX2 cDNA that is tagged at the C-terminal end with 3 copies of Tag:FLAG.

(Shen et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Hsap\TBX2
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  cancer
      CEA
      (Shen et al., 2014)
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      model of  cancer
      is NOT ameliorated by TimpUAS.cPa
      (Shen et al., 2014)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of Hsap\TBX2Scer\UAS.T:Zzzz\FLAG, under the control of Scer\GAL4dpp.PU throughout development, or in combination with gal80[ts] to restrict expression to the larval stage results in some cells crossing the anterior-posterior compartment border and displaying actin protrusions after 15h of gal80[ts]-regulated expression; expression under the control of Scer\GAL4C-765 results in a proportion of disc cells becoming embedded in the extracellular matrix and basally delaminating, as cells extrude from the epithelium and accumulate on the other side of the basal membrane, as compared to controls.

      (Shen et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      The co-expression of TimpScer\UAS.cPa does not significantly suppress the long distance cell migration and scattering, and the basal delamination of cells resulting from the expression of Hsap\TBX2Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4dpp.PU and Scer\GAL4C-765, respectively.

      (Shen et al., 2014)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hsap\TBX2Scer\UAS.T:Zzzz\FLAG
      Hsap\TBX2UAS.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (1)