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General Information
Symbol
Dmel\dncΔ143
Species
D. melanogaster
Name
FlyBase ID
FBal0325387
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

2219 bp deletion resulting from the imprecise excision of P{EP}dncEP973. The deleton removes sequences from -2148 to +71 relative to the transcription start site of dnc-RA. Deletion was mapped against the dnc-RA tss in R6.16 .

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Deletion in the first exon of the dnc[RA] transcript (extending from -2148 to +71 relative to the transcription start site of dnc[RA]). This reduces the level of the dnc[RA] transcript almost to zero, slightly reduces dnc[RL] expression and does not affect the expression of the other dnc transcript groups.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Upon initial exposition, dncΔ143 adult mutants show ethanol sensitivity that is comparable to wild-type controls but exhibit reduced development of tolerance (= increase in resistance during re-exposition) as well as decreased heat-induced ethanol resistance normally observed in wild-type flies.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
NOT Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The reduced heat-induced ethanol (EtOH) resistance characteristic for hangAE10 mutant adults is not exacerbated further by combination with dncΔ143 which on its own also lead to a decrease in the heat > ethanol cross-tolerance.

hangAE10;dncΔ143 double mutant adult flies display normal ethanol tolerance development upon re-exposition to EtOH, unlike each of the single mutants that show significantly reduced tolerance buildout.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The reduced ability of dncΔ143 mutant adults to develop tolerance to ethanol upon re-exposition is rescued by expression of dncScer\UAS.all when driven by either Scer\GAL4dnc.RA (alone or combined with Scer\GAL80ple.PK to restrict the expression pattern) or Scer\GAL4NP6510 but not when driven by Scer\GAL4GMR58E02. The tolerance buildout can also be rescued by Scer\GAL4dnc.RA-driven expression of dncScer\UAS.RA.T:Avic\GFP-EGFP but not dncScer\UAS.RG.T:Avic\GFP-EGFP or dncScer\UAS.RL.T:Avic\GFP-EGFP.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (1)